Alexa Fluor® 647 anti-Cytokeratin 19 Antibody

Pricing & Availability
Clone
A53-B/A2 (See other available formats)
Regulatory Status
RUO
Other Names
Keratin 19, Keratin 19 type 1 cytoskeletal
Isotype
Mouse IgG2a, κ
A53-BA2_AF647_Cytokeratin19_Antibody_IF_070114
MCF-7 cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes at 37°C, permeabilized with 0.5% Triton X-100 buffer for 10 minutes at room temperature, blocked for 30 minutes at room temperature with 5% fetal bovine serum (FBS), then stained with 5 µg/mL anti-Cytokeratin 19 (clone A53-B/A2) Alexa Fluor® 647 (red). Nuclei were counterstained with DAPI (blue). Images were acquired with a 60X objective.
  • A53-BA2_AF647_Cytokeratin19_Antibody_IF_070114
    MCF-7 cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes at 37°C, permeabilized with 0.5% Triton X-100 buffer for 10 minutes at room temperature, blocked for 30 minutes at room temperature with 5% fetal bovine serum (FBS), then stained with 5 µg/mL anti-Cytokeratin 19 (clone A53-B/A2) Alexa Fluor® 647 (red). Nuclei were counterstained with DAPI (blue). Images were acquired with a 60X objective.
Compare all formats See Alexa Fluor® 647 spectral data
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628506 100 µg $335.00
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Description

Cytokeratin 19, also known as keratin 19, is a type I intermediate filament protein with a molecular weight of approximately 40-44 kD. Cytokeratin 19 is a heterotetramer composed of two type I and two type II keratin subunits. Unlike other cytokeratins, cytokeratin 19 lacks a C-terminal non-helical extension. This cytokeratin is widely expressed in the periderm (transient superficial layer enveloping developing epidermis), muscle, intestine, bile duct, esophagus, stomach, and thymus. Cytokeratin 19 can be upregulated by vitamin A and is thought to play a critical role in embryogenesis. Cytokeratin 19 intereacts with the pinin protein and has been shown to be modified by phosphorylation (Ser10, Ser35).

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human mammary carcinoma cell line MCF-7
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemistry of paraffin-embedded sections, immunocytochemistry, and ELISA.

Application References

(PubMed link indicates BioLegend citation)
  1. Karsten U, et al. 1985. Eur. J. Cancer Clin. Oncol. 21:733.
  2. Nishikata T,et al. Anticancer Res. 33:2867. PubMed
Product Citations
  1. Seaman S, et al. 2017. Cancer Cell. 31:501. PubMed
  2. Pham K, et al. 2016. Am J Pathol. 186: 1537-1546. PubMed
  3. Lai BFL, et al. 2021. Nature Protocols. 16(4):2158-2189. PubMed
RRID
AB_2563651 (BioLegend Cat. No. 628506)

Antigen Details

Structure
Type I intermediate filament protein, contains three coiled-coil domains, lacks C-terminal non-helical extension found in other keratins, approximately 40-44 kD. Cytokeratin 19 is a heterotetramer composed of two type I and two type II keratin subunits.
Distribution

Expressed in the periderm (transient superficial layer enveloping developing epidermis), also expressed in muscle, intestine, bile duct, esophagus, stomach, and thymus. Upregulated by vitamin A.

Function
Intermediate filament protein involved with the cytoskeleton. May play a critical role in embryogenesis
Interaction
Interacts with pinin
Modification
Phosphorylation (Ser10, Ser35)
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Antigen References

1. Bader BL, et al. 1986. EMBO J. 5:1865.
2. Eckert RL. 1988. Proc. Natl. Acad. Sci. 85:1114.
3. Stasiak PC and Lane EB. 1987 Nucleic Acids Res. 15:10058.

Gene ID
3880 View all products for this Gene ID
UniProt
View information about Cytokeratin 19 on UniProt.org
Go To Top Version: 2    Revision Date: 08/08/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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