Alexa Fluor® 647 anti-Cytokeratin (pan reactive) Antibody

Pricing & Availability
Clone
C-11 (See other available formats)
Regulatory Status
RUO
Other Names
Cytokeratin, Keratin
Isotype
Mouse IgG1, κ
1_C-11_A647_Cytokeratin_Antibody_ICC_080619
MCF-7 cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes at 37°C, permeabilized with 0.5% TritonX-100 buffer for 10 minutes at room temperature, and blocked for 30 minutes at room temperature with 5% fetal bovine serum (FBS). Then the cells were stained with 5 µg/mL anti-cytokeratin (pan reactive) (clone C-11) Alexa Fluor® 647 (red). Nuclei were counterstained with DAPI (blue). Images were acquired with a 60X objective.
  • 1_C-11_A647_Cytokeratin_Antibody_ICC_080619
    MCF-7 cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes at 37°C, permeabilized with 0.5% TritonX-100 buffer for 10 minutes at room temperature, and blocked for 30 minutes at room temperature with 5% fetal bovine serum (FBS). Then the cells were stained with 5 µg/mL anti-cytokeratin (pan reactive) (clone C-11) Alexa Fluor® 647 (red). Nuclei were counterstained with DAPI (blue). Images were acquired with a 60X objective.
  • 2_C-11_Alexa-Fluor-647_Antibody_051622
    MCF7 cells were fixed and permeabilized using Cyto-Fast Fix/Perm Buffer (Cat. No. 426803) and intracellularly stained with Alexa Fluor® 647 anti-Cytokeratin (Cat. No. 628604) (Filled histogram), or with Alexa Fluor® 647 isotype control (Cat. No. 400135) (Open histogram).
  • 3_C-11_Alexa-Fluor-647_Antibody_Im2_051622
    MCF7 cells (positive control, solid histogram), and Jurkat cells (negative control, open histogram) were fixed and permeabilized using Cyto-Fast Fix/Perm Buffer (Cat. No. 426803) and intracellularly stained with Alexa Fluor® 647 anti-Cytokeratin (Cat. No. 628604).
  • 4_37_Mouse_Thymus_CD206_Cytokeratin
    Confocal image of C57BL/6 mouse thymus sample acquired using the IBEX method of highly multiplexed antibody-based imaging: CD206 (cyan) in Cycle 4 and Cytokeratin (purple) in Cycle 5. Tissues were prepared using ~1% (vol/vol) formaldehyde and a detergent. Following fixation, samples are immersed in 30% (wt/vol) sucrose for cryoprotection. Images are courtesy of Drs. Andrea J. Radtke and Ronald N. Germain of the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
Compare all formats See Alexa Fluor® 647 spectral data
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628604 100 µg $347.00
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Description

Cytokeratins are intermediate filament proteins that are widely expressed in many tissues. These proteins are vital components of the cytoskeleton and interact with a number of other proteins. Cytokeratins are usually found in the cytoplasm,although some cytokeratins can be found in the nucleus and the nucleolus.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Keratin enriched fraction from human epidermoid carcinoma.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
ICFC - Verified
SB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

The C-11 monoclonal antibody reacts with a conserved epitope of human cytokeratin 4, 5, 6, 8, 10, 13, and 18. This antibody has been shown to be useful for Western blotting and has also been reported to be useful for immunoprecipitation, immunohistochemistry (paraffin sections), immunocytochemistry, and spatial biology (IBEX)7,8.

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References

(PubMed link indicates BioLegend citation)
  1. Kovarik J. 1988. Int. J. Cancer Suppl. 3:50.
  2. Bartek J, et al. 1991. J. Pathol. 164:215.
  3. Chernyavsky A I, 2007. J. Biol. Chem. doi:10.1074/jbc.M611365200. PubMed
  4. Comito G, et al. 2012. Cancer Lett. 324:31. PubMed
  5. Vojt-òsek B, et al. 1989. Folia Biol (Praha). 35:373. (reactivity with rat, dog, sheep, pig, cow)
  6. Dekaney CM, et al. 2005. Gastroenterology 129:1567. (ICC)
  7. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  8. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Roy S, et al. 2019. J Cell Biochem. 120:4804. PubMed
  2. Hendricks A, et al. 2021. Front Oncol. 11:646885. PubMed
  3. Hendricks A, et al. 2020. Cancers (Basel). 12:00. PubMed
  4. Vishnoi M, et al. 2019. Mol Oncol. 13(9): 1913. PubMed
RRID
AB_2563652 (BioLegend Cat. No. 628604)

Antigen Details

Structure
Intermediate filament protein, contains three coiled-coil domains. Cytokeratins exist as heterotetramers composed of two type I and two type II keratin subunits
Distribution

Cytokeratins are widely expressed proteins usually found in the cytoplasm although some cytokeratins can also be found in the nucleus and the nucleolus

Function
Intermediate filament protein involved with the cytoskeleton.
Interaction
Most cytokeratins interact with a number of other proteins such as 14-3-3, EGF receptor, and many others
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
3875 View all products for this Gene ID
UniProt
View information about Cytokeratin pan reactive on UniProt.org
Go To Top Version: 6    Revision Date: 05/16/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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