Alexa Fluor® 647 anti-human CD56 (NCAM) Antibody

Pricing & Availability
Clone
A19063A (See other available formats)
Regulatory Status
RUO
Other Names
Leu-19, NKH1, NCAM-1
Isotype
Mouse IgG2b, κ
A19063A_ALEXA-647_CD56_IHC-P-1_091523
IHC staining of anti-human CD56 (clone A19063A) Alexa Fluor® 647 on formalin-fixed paraffin-embedded human brain tissue. After antigen retrieval using 1X Citrate Buffer (Cat. No. 420902), the tissue was incubated with 10 µg/mL of anti-human CD56 (clone A19063A) Alexa Fluor® 647 (red) at 4°C overnight. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801). The image was captured with a 10X objective.
  • A19063A_ALEXA-647_CD56_IHC-P-1_091523
    IHC staining of anti-human CD56 (clone A19063A) Alexa Fluor® 647 on formalin-fixed paraffin-embedded human brain tissue. After antigen retrieval using 1X Citrate Buffer (Cat. No. 420902), the tissue was incubated with 10 µg/mL of anti-human CD56 (clone A19063A) Alexa Fluor® 647 (red) at 4°C overnight. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801). The image was captured with a 10X objective.
  • A19063A_ALEXA-647_CD56_IHC-P-2_091523
    IHC staining of anti-human CD56 (clone A19063A) Alexa Fluor® 647 on formalin-fixed paraffin-embedded human kidney tissue (negative control). After antigen retrieval using 1X Citrate Buffer (Cat. No. 420902), the tissue was incubated with 10 µg/mL of anti-human CD56 (clone A19063A) Alexa Fluor® 647 (red) at 4°C overnight. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801). The image was captured with a 10X objective.
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability
380703 25 µg $105.00
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380704 100 µg $205.00
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Description

CD56 is a single transmembrane glycoprotein also known as NCAM (neural cell adhesion molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK and NKT cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparan sulfate.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. 

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
RRID
AB_3662279 (BioLegend Cat. No. 380703)
AB_3662279 (BioLegend Cat. No. 380704)

Antigen Details

Structure
Ig superfamily, single transmembrane or GPI-anchored glycoprotein
Distribution

NK cells, T subset, neural tissue, some LGL and myeloid leukemias

Function
Adhesion
Ligand/Receptor
Heparan sulfate
Cell Type
B cells, Leukemia, Mesenchymal Stem Cells, Neurons, NK cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Laroni A, et al. 2016. J Autoimmun. 72:8-18. PubMed
  2. Pachnio A, et al. 2016. PLoS Pathog. 12:e1005832. PubMed
  3. Wouters K, et al. 2017. Sci Rep. 7:42665. PubMed

 

Gene ID
4684 View all products for this Gene ID
UniProt
View information about CD56 on UniProt.org
Go To Top Version: 1    Revision Date: 09/15/2023

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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