- Clone
- A20004B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Cyclin Dependent Kinase 1, Cdk1, CDC28A, P34CDC2, P34 Protein Kinase
- Isotype
- Mouse IgG2b, κ
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HeLa cells untreated (negative control, open histogram) or treated with 4mM Hydroxyurea (positive control, filled histogram) were fixed and permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401), and intracellularly stained with Alexa Fluor® 647 anti-human cdc2 Phospho (Thr14) (clone A20004B), or with Alexa Fluor® 647 mouse IgG2b, κ isotype control (open histogram, dashed line) (representative histogram for treated and untreated cells) (Cat. No. 400330).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 947405 | 25 tests | $198.00 | |||
| 947406 | 100 tests | $469.00 | |||
Cyclin-dependent kinase 1 (Cdk1), also known as cell division control protein 2 (cdc2), is a key regulatory kinase essential for cell growth, proliferation, cell cycle progression, and early embryonic development. It is responsible for driving cells through G2 phase into M phase. Prior to mitosis, cdc2, along with its binding partner cyclin B1, exists in an inactive state when phosphorylated on Thr14 and Tyr15 by PKMYT1 (MYT1) and WEE1. This B1-cdc2 complex is activated during G2 and early mitosis by cdc25-mediated dephosphorylation. During mitosis, cdc2 inhibits DNA re-replication and subsequent polyploidy and cell senescence through the sequestering of cyclin A2 and suppression of the formation of CDK2-cyclin A complexes. cdc2 can function as a substitute for other cdc family members and drive the progression of the cell cycle in the event of their loss. cdc2 functions as a positive regulator of global translation, protein synthesis, and genome stability and participates in DNA damage repair, checkpoint activation, and DNA replication fork progression. It is overexpressed in several cancers including liver, breast, and colorectal cancers and has been implicated in the promotion of tumorigenesis.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide corresponding to human cdc2 phosphorylated at threonine 14
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
This clone is predicted to react with mouse cdc2 when phosphorylated at threonine 14 due to complete sequence homology between immunizing peptide and the cdc2 mouse orthologue.
This clone was tested for ICC using lambda protein phosphatase analysis and HeLa cells fixed with methanol or 4% PFA followed by permeabilization with either methanol or Triton X-100. Only PFA fixation followed by methanol permeabilization produced phospho-specific staining of cdc2. - RRID
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AB_2936756 (BioLegend Cat. No. 947405)
AB_2936756 (BioLegend Cat. No. 947406)
Antigen Details
- Structure
- cdc2 is a 297 amino acid protein with a predicted molecular weight of 34 kD.
- Distribution
-
Ubiquitously expressed/ Mitochondria, nucleus, cytoskeleton, cytoplasm
- Function
- Serine/threonine-protein kinase/ Cell Cycle
- Interaction
- Cyclin B1, Cyclin A, PKMYT1 (MYT1)
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, Signal Transduction
- Molecular Family
- Protein Kinases/Phosphatase
- Antigen References
-
- Chow J, et al. 2011. Mol. Cell Biol. 31:1478-91.
- Diril M, et al. 2012. PNAS. 109:3826-31.
- Haneke K, et al. 2020. J. Cell. Biol. 219: e201906147.
- Liao H, et al. 2017. Oncotarget. 8:90662-73.
- Prevo R, et al. 2018. Cell Cycle. 17:1513-23.
- Gene ID
- 983 View all products for this Gene ID
- UniProt
- View information about cdc2 on UniProt.org
Other Formats
View All cdc2 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human cdc2 Phospho (Thr14) | A20004B | WB,ICC,ICFC |
| Alexa Fluor® 647 anti-human cdc2 Phospho (Thr14) | A20004B | ICFC |
| PE anti-human cdc2 Phospho (Thr14) | A20004B | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human cdc2 Phospho (Thr14)
Whole cell extracts (15 µg total protein) from serum-starved... 
HeLa cells treated with 4 mM of hydroxyurea for 20 hours (fi... 
HeLa cells treated with LPP overnight at 4°C (negative) (pan... -
Alexa Fluor® 647 anti-human cdc2 Phospho (Thr14)
HeLa cells untreated (negative control, open histogram) or t... -
PE anti-human cdc2 Phospho (Thr14)
HeLa cells treated with 4mM Hydroxyurea for 20 hours were fi... 
Hydroxyurea-treated HeLa cells (filled histogram, positive c...