Alexa Fluor® 647 anti-human Perforin Antibody

Pricing & Availability
Clone
B-D48 (See other available formats)
Regulatory Status
RUO
Other Names
PRF1, P1, PFP, HPLH2
Isotype
Mouse IgG1
B-D48_A647_Perforin_Antibody_101018
Human peripheral blood lymphocytes were surface stained with CD8 PE, fixed with fixation buffer, permeabilized with intracellular staining permeabilization wash buffer and then intracellularly stained with Perforin (clone B-D48) Alexa Fluor® 647 (left), or mouse IgG1, κ Alexa Fluor® 647 isotype control (right).
  • B-D48_A647_Perforin_Antibody_101018
    Human peripheral blood lymphocytes were surface stained with CD8 PE, fixed with fixation buffer, permeabilized with intracellular staining permeabilization wash buffer and then intracellularly stained with Perforin (clone B-D48) Alexa Fluor® 647 (left), or mouse IgG1, κ Alexa Fluor® 647 isotype control (right).
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability
353321 25 tests $141.00
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353322 100 tests $352.00
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Description

Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Product Citations
  1. Vardam-Kaur T, et al. 2021. Oncotarget. 12:2051. PubMed
RRID
AB_2783256 (BioLegend Cat. No. 353321)
AB_2783256 (BioLegend Cat. No. 353322)

Antigen Details

Structure
70 kD
Distribution

CTL, NK (cytoplasmic granules)

Function
Mediates targeted cell lysis.
Cell Type
NK cells, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Lieberman J. 2003. Nat. Rev. Immunol. 3:361.
2. Trapani J, et al. 2002. Nat. Rev. Immunol. 2:735.

Gene ID
5551 View all products for this Gene ID
UniProt
View information about Perforin on UniProt.org
Go To Top Version: 1    Revision Date: 10/10/2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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