- Clone
- A20005D (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Linker For Activation Of T Cells; P36-38
- Isotype
- Mouse IgG2b, κ
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Jurkat cells untreated (negative control, open histogram) or treated with 5 mM of H2O2 for 3 minutes (positive control, solid histogram) were fixed and permeabilized using True Phos Perm Buffer (Cat No. 425401) and intracellularly stained with Alexa Fluor® 647 anti-LAT Phospho (Tyr 171) (clone A20005D), or Alexa Fluor® 647 Mouse IgG2b, κ isotype control (open histogram, dashed line)(representative histogram for untreated and H2O2 treated Jurkat cells) (Cat No. 400330). -
Peripherical blood mononuclear cells (PBMC) untreated (left), or treated with 5mM of H2O2 for 3 minutes (right) were fixed and permeabilizated using True Phos Perm Buffer (Cat No. 425401), and then intracellular stained with Alexa Fluor® 647 anti-LAT Phospho (Tyr 171) (clone A20005D), and Alexa Fluor® 488 anti-CD3 (Cat No. 317310).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 946603 | 25 tests | $198.00 | |||
| 946604 | 100 tests | $469.00 | |||
LAT, or Linker for Activation of T cells, is a transmembrane protein expressed mainly in T cells and a limited number of other immune cells such as natural killer cells, mast cells, and immature B cells. As an adaptor protein, phosphorylated LAT facilitates the recruitment of other signaling proteins and functions as a nucleating site for multiprotein signaling complexes. These signaling complexes can propagate TCR, or T Cell antigen Receptors, and activate downstream effectors, which trigger T cell proliferation or cytokine expression.
LAT (Tyr171) is one of the nine tyrosines in LAT conserved between human, mice, and rat. LAT (Tyr171) is necessary for the activation of PI 3-kinase, a protein that regulates the recruitment of other proteins that facilitate in T-cell receptor signal transduction such as PLC-gamma1 and Sos1. When LAT (Tyr171) and (Tyr191) is phosphorylated by ZAP-70, Gads can bind directly to LAT (Tyr171) and (Tyr191) and mediate the recruitment of SLP-76.
Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide corresponding to human LAT phosphorylated at Tyr 171
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
When this clone is used in WB at a dilution lower than 1:1000 it may show non-specific bands at 50 kD and above.
This clone has been tested by ICC on H2O2 treated and untreated Jurkat, with three fix and permeabilization methods (100% methanol, 4% PFA plus methanol and 4% PFA plus Triton X-100). All three methods can be used for ICC staining, but the PFA plus Triton X-100 fixation/permeabilization method produced the best staining.
This clone has only been tested on True-PhosTM perm buffer (Cat. No. 425401) by ICFC - RRID
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AB_2927971 (BioLegend Cat. No. 946603)
AB_2927971 (BioLegend Cat. No. 946604)
Antigen Details
- Structure
- LAT is a 262 amino acid protein with a predicted molecular weight of 36-38 kD.
- Distribution
-
Plasma membrane/T Cells
- Function
- Plasma membrane/T Cells
- Cell Type
- Mast cells, NK cells, T cells
- Biology Area
- Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Phospho-Proteins
- Antigen References
-
- Balagopalan L. et al., 2010, Cold Spring Harb Perspect Biol., 2(8): a005512
- Balagopalan L. et al., 2020, PLoS One, 15(2): e0229036
- Lin J. and Weiss A., 2001, J Biol Chem., 276(31): 29588-95
- Paz et. al., 2001, Biochem J., 356, 461-71
- Zhang W. et al., 2000, J Biol Chem., 275(30): 23355-61
- Gene ID
- 27040 View all products for this Gene ID
- UniProt
- View information about LAT Phospho Tyr171 on UniProt.org
Other Formats
View All LAT Phospho (Tyr171) Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-LAT Phospho (Tyr171) | A20005D | WB,ICC,ICFC |
| Alexa Fluor® 647 anti-LAT Phospho (Tyr171) | A20005D | ICFC |
| PE anti-LAT Phospho (Tyr171) | A20005D | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-LAT Phospho (Tyr171)
Whole cell extracts (15 μg protein) from serum-starved Jurka... 
Serum-starved Jurkat cells untreated (lower) and treated (up... 
Jurkat cells treated (filled histogram, positive control) wi... -
Alexa Fluor® 647 anti-LAT Phospho (Tyr171)
Jurkat cells untreated (negative control, open histogram) or... 
Peripherical blood mononuclear cells (PBMC) untreated (left)... -
PE anti-LAT Phospho (Tyr171)
Jurkat cells untreated (negative control, open histogram), o... 
Peripherical blood mononuclear cells (PBMC) untreated (left)...