- Clone
- 14E6-4E6 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Protein Lin-28 Homolog A, Lin-28A, CSDD1, ZCCHC1, Zinc Finger CCHC Domain-Containing Protein 1
- Isotype
- Mouse IgG2a, κ
-
NTERA2 cells (positive target) were fixed with Fixation Buffer (Cat. No. 420801), permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401), and intracellularly stained with Alexa Fluor® 647 anti-LIN28A (clone 14E6-4E6) (filled histogram) or Alexa Fluor® 647 Mouse IgG2a, κ isotype control (Cat. No. 400240) (open histogram) followed by PE goat anti-mouse IgG (Cat. No. 405307). -
HeLa cells (negative target) were fixed with Fixation Buffer (Cat. No. 420801), permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401), and intracellularly stained with Alexa Fluor® 647 anti-LIN28A (clone 14E6-4E6), (filled histogram) or Alexa Fluor® 647 Mouse IgG2a, κ isotype control (Cat. No. 400240) (open histogram).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 657805 | 25 tests | $183.00 | |||
| 657806 | 100 tests | $382.00 | |||
LIN28A is a highly conserved RNA-binding protein and reprogramming factor that plays important roles in development, self-renewal of stem cells, glucose metabolism and tumorigenesis. Lin28A modulates not only miRNAs, particularly the let-7 family miRNAs, but also the translation of mRNAs that are important for cell growth and metabolism. LIN28A/B has been observed to be overexpressed in a wide range of solid tumors and hematological malignancies, and has emerged as a new therapeutic target candidate for cancer.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human Lin28A from E. coli
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
This clone is specific for Lin28A does not recognize Lin28B.
- RRID
-
AB_2924622 (BioLegend Cat. No. 657805)
AB_2924622 (BioLegend Cat. No. 657806)
Antigen Details
- Structure
- 209 amino acids, predicted molecular weight of 28 kD with the characteristic of a cold shock domain (CSD) and two tandem Cys-Cys-His-Cys (CCHC)-type zinc-binding motifs (CCHCx2)
- Distribution
-
Cytoplasm and nucleus; localizes to cytoplasmic processing bodies and stress granules
- Function
- Development, self-renewal of stem cells, glucose metabolism and tumorigenesis; regulates Let-7 family miRNA, translation of mRNA
- Interaction
- EIF3S2, NCL and ZCCHC11/TUT4
- Cell Type
- Embryonic Stem Cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics, Immunology, Protein Synthesis, Signal Transduction, Stem Cells
- Antigen References
-
1. Shyh-Chang N, et al. 2013. Cell Stem Cell. 12:395.
2. Thornton JE, et al. 2012. Trends Cell Biol. 22:474.
3. Huang Y. 2012. Wiley Interdiscip. Rev. RNA. 3:483.
4. Cho J, et al. 2012. Cell. 151:765.
5. Li N, et al. 2012. J. Biol. Chem. 287:17386.
6. Zhu H, et al. 2011. Cell 147:81. - Gene ID
- 79727 View all products for this Gene ID
- UniProt
- View information about Lin28A on UniProt.org
Other Formats
View All Lin28A Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Lin28A | 14E6-4E6 | WB,ICC,ICFC |
| Alexa Fluor® 647 anti-Lin28A | 14E6-4E6 | ICFC |
| Alexa Fluor® 488 anti-Lin28A | 14E6-4E6 | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Lin28A
Total cell lysate from HeLa cells (lane 1, 15 µg), NTERA2 (l... 
Whole cell lysates (15 µg protein) from HeLa (negative contr... 
NTERA‐2 cells were fixed with 4% paraformaldehyde (PFA) for ... 
HeLa cells (negative target) were fixed with Fixation Buffer... 
NTERA2 cells (positive target) were fixed with Fixation Buff... -
Alexa Fluor® 647 anti-Lin28A
HeLa cells (negative target) were fixed with Fixation Buffer... 
NTERA2 cells (positive target) were fixed with Fixation Buff... -
Alexa Fluor® 488 anti-Lin28A
HeLa cells (negative target) were fixed with Fixation Buffer... 
NTERA2 cells (positive target) were fixed with Fixation Buff...