Alexa Fluor® 647 anti-mouse CD195 (CCR5) Antibody

Pricing & Availability
Clone
HM-CCR5 (See other available formats)
Regulatory Status
RUO
Other Names
CCR5, C-C chemokine receptor type 5, HIV-1 fusion co-receptor
Isotype
Armenian Hamster IgG
HM-CCR5_Alx647_090407
Mouse CCR5 transfected cells stained with HM-CCR5 Alexa Fluor® 647
  • HM-CCR5_Alx647_090407
    Mouse CCR5 transfected cells stained with HM-CCR5 Alexa Fluor® 647
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability
107010 100 µg $265.00
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Description

CD195 is a 45 kD chemokine receptor also known as CCR5. CD195 is a seven transmembrane-spanning G protein-associated molecule expressed on macrophages, a T cell subset, and in the heart, liver, and spleen. CD195 regulates lymphocyte chemotaxis and transendothelial migration during inflammatory processes. CD195 interacts with several ligands including RANTES, MCP-1, MIP-1α, and MIP-1β.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

CCR5 is expressed at low density on activated cells. For successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 107006) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 107004) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)).

Application References

(PubMed link indicates BioLegend citation)
  1. Mao A, et al. 2005. J. Immunol. 175:5146. (FC) PubMed
  2. Ishida Y, et al. 2007. Am J Pathol.170:843.(FC) PubMed
  3. Zeiser Z, et al. 2008. Blood 111:453. (FC) PubMed
  4. Sharma R, et al. 2009. J. Immunol.. 183:3212 (FC) PubMed
  5. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Tofukuji S, et al. 2012. J Immunol. 188:4846. PubMed
RRID
AB_830709 (BioLegend Cat. No. 107010)

Antigen Details

Structure
β-chemokine receptor, 45 kD
Distribution

Macrophages, T cell subset, heart, spleen, liver

Function
Lymphocyte chemotaxis and transendothelial migration during inflammation, signaling through seven transmembrane-spanning G proteins
Ligand/Receptor
RANTES, MCP-1, MIP-1α, and MIP-1β
Cell Type
Dendritic cells, Macrophages, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Napolitano M, et al. 1990. J. Exp. Med. 172:285.
3. Meyer A, et al. 1996. J. Biol. Chem. 271:14445.
4. Boring, et al. 1996. J. Biol. Chem. 271:7551.

Gene ID
12774 View all products for this Gene ID
UniProt
View information about CD195 on UniProt.org
Go To Top Version: 2    Revision Date: 06/27/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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