- Clone
- 129C1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- BST2, tetherin, HM1.2 antigen, bone marrow stromal antigen 2, PDCA-1
- Isotype
- Rat IgG2b, κ
-
C57BL/6 mouse splenocytes stained with 129C1 Alexa Fluor® 647 and CD45R/B220 (RA3-6B2) PE -
C57BL/6 mouse splenocytes stained with rat IgG2b Alexa Fluor® 647 isotype control and CD45R/B220 (RA3-6B2) PE
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 127105 | 25 µg | $141.00 | |||
| 127106 | 100 µg | $293.00 | |||
CD317, known as BST2, tetherin, HM1.2 antigen, bone marrow stromal antigen 2, or PDCA-1, is type II transmembrane glycoprotein with a molecular mass of 29-33 kD. It is predominantly expressed on Type I IFN-producing cells (IPCs) in naïve mice, but is up-regulated on most cell types following stimulation with type I IFNs and IFN-gamma. It is highly expressed on terminally differentiated normal plasmacytoid dendritic cells and some tumor cells, such as multiple myeloma, renal cell carcinoma, and melanoma cells. BST2 was recently identified as an IFN-induced cellular response factor that blocks release of HIV-1 and other retroviruses from infected cells. BST2 has been found to be the natural ligand of ILT7 in human model.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse plasmacytoid dendritic cells (DCs)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
-
Application References
(PubMed link indicates BioLegend citation) -
- Blasius AL, et al. 2006. J. Immunol. 177:3260.
- Product Citations
-
- RRID
-
AB_2228222 (BioLegend Cat. No. 127105)
AB_2228222 (BioLegend Cat. No. 127106)
Antigen Details
- Structure
- Type II transmembrane glycoprotein with a molecular mass of 29-33 kD.
- Distribution
- Expressed on type I IFN-producing cells, plasmacytoid dendritic cells, and neoplastic B cells, such as multiple myeloma.
- Function
- Recently identified as antiviral protein that blocks the release of nascent retrovirus or other particles from infected cells.
- Cell Type
- Dendritic cells, B cells
- Biology Area
- Immunology, Innate Immunity, Costimulatory Molecules
- Molecular Family
- CD Molecules
- Antigen References
-
1. Douglas JL, et al. 2009. J Virol. 83:7931.
2. Cao W, et al. 2009. J. Exp. Med. 206:1603.
3. Neil SJ, et al. 2008. Nature 451:425. - Gene ID
- 69550 View all products for this Gene ID
- UniProt
- View information about CD317 on UniProt.org
Other Formats
View All CD317 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse CD317 (BST2, PDCA-1) | 129C1 | FC,IP |
| PE anti-mouse CD317 (BST2, PDCA-1) | 129C1 | FC |
| Pacific Blue™ anti-mouse CD317 (BST2, PDCA-1) | 129C1 | FC |
| Alexa Fluor® 647 anti-mouse CD317 (BST2, PDCA-1) | 129C1 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-mouse CD317 (BST2, PDCA-1)
SJL mouse splenocytes double stained with 129C1 PE and CD11c... 
SJL mouse splenocytes double stained with rat IgG2b isotype ... -
PE anti-mouse CD317 (BST2, PDCA-1)
SJL mouse splenocytes double stained with 129C1 PE and CD11c...
SJL mouse splenocytes double stained with rat IgG2b isotype ... -
Pacific Blue™ anti-mouse CD317 (BST2, PDCA-1)
C57BL/6 splenocytes stained with 129C1 Pacific Blue™ a... 
C57BL/6 splenocytes stained with rat IgG2b Pacific Blue&trad... -
Alexa Fluor® 647 anti-mouse CD317 (BST2, PDCA-1)
C57BL/6 mouse splenocytes stained with 129C1 Alexa Fluor&re... 
C57BL/6 mouse splenocytes stained with rat IgG2b Alexa Fluo...