Alexa Fluor® 647 anti-mouse CD38 Antibody

Pricing & Availability
Clone
90 (See other available formats)
Regulatory Status
RUO
Other Names
T10
Isotype
Rat IgG2a, κ
A-90_Alx647_011108
C57BL/6 mouse splenocytes stained with 90 Alexa Fluor® 647
  • A-90_Alx647_011108
    C57BL/6 mouse splenocytes stained with 90 Alexa Fluor® 647
  • B-90_A647_CD38_Antibody_IHCF_051419
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS+ rat/mouse/hamster serum for 30 minutes at room temperature. Then the section was stained with 5 µg/ml of Alexa Fluor® 647 anti-mouse CD38 (red) (Clone 90), 5 µg/ml Alexa Fluor® 488 anti-mouse CD3ε (green) (clone 145-2C11), and 5 µg/ml of Alexa Fluor® 594 anti-mouse F4/80 Antibody (blue) (Clone BM8) overnight at 4°C. The image was captured by 10X objective.
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability
102716 100 µg $229.00
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Description

CD38 is a 45 kD type II transmembrane glycoprotein also known as T10. It is an ADP-ribosyl hydrolase expressed at variable levels on hematopoietic cells and in some non-hematopoietic tissues (such as brain, muscle, and kidney). In humans, it is expressed at high levels on plasma cells and activated T and B cells, natural killer (NK) lymphocytes, myeloblasts, and erythroblasts. By functioning as both a cyclase and a hydrolase, CD38 mediates lymphocyte activation, adhesion, and the metabolism of cADPR and NAADP. CD31 is the ligand of CD38.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse bone marrow pre-B cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 10 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Product Citations
  1. Enterina JR, et al. 2022. Cell Rep. 38:110512. PubMed
  2. Gon Y, et al. 2023. PLoS One. 18:e0279389. PubMed
  3. Yazicioglu YF, et al. 2023. Nat Immunol. 24:991. PubMed
  4. Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed
  5. Nabar NR, et al. 2022. Autophagy. 18:204. PubMed
  6. Tan AHY, et al. 2019. Front Immunol. 10:1351. PubMed
  7. Ray A, et al. 2015. PLoS One. 10: 0137314. PubMed
  8. Zhang Y, et al. 2018. J Exp Med. 215:1227. PubMed
  9. Lindhorst A, et al. 2021. Cell Death Dis. 12:579. PubMed
  10. Hong JP, et al. 2020. Cell Reports Medicine. 1(3):100035. PubMed
RRID
AB_2073334 (BioLegend Cat. No. 102716)

Antigen Details

Structure
ADP-ribosyl hydrolase, 42 kD
Distribution

B cells, NK cells, T cell subset, brain, muscle, kidney

Function
Regulation of cellular activation/proliferation, adhesion, metabolism of cADPR and NAADP
Ligand/Receptor
CD31, hyaluronic acid
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Shubinsky G, et al. 1997. Immunity 7:315.
3. Cesano A, et al. 1998. J. Immunol. 160:1106.
4. Cockayne DA, et al. 1998. Blood 92:1324.

Gene ID
12494 View all products for this Gene ID
UniProt
View information about CD38 on UniProt.org
Go To Top Version: 2    Revision Date: 06/27/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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