Alexa Fluor® 647 anti-mouse CD43 Activation-Associated Glycoform Antibody

Pricing & Availability
Clone
1B11 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, gp115
Isotype
Rat IgG2a, κ
1-1B11_Alx647_021808
C57BL/6 mouse splenocytes stained with 1B11 Alexa Fluor® 647
  • 1-1B11_Alx647_021808
    C57BL/6 mouse splenocytes stained with 1B11 Alexa Fluor® 647
  • 2-1B11_A647_CD43_Antibody_2_IHCF_061218
    3 Day 4T1 cells induced BALB/c frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 1 hour at room temperature. Then the section was stained with 5 µg/ml of CD3ε (clone 500A2) Alexa Fluor® 594 (blue), CD43 (clone 1B11) Alexa Fluor® 647 (red) and B220 (clone RA3-6B2) Alexa Fluor® 488 (green) at 4°C overnight. The image was scanned with a 10X object and stitched with MetaMorph® software.
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability
121212 100 µg $229.00
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Description

CD43, also known as Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, and gp115, is a large single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites. Due to variable glycosylation and sialylation, two isoforms of CD43 have been identified. The 115 kD glycoform of CD43 is expressed on most hematopietic cells including T lymphocytes, NK cells, monocytes, granulocytes, platelets, and CD5+ B cells. It is not present on resting B cells and erythrocytes. While the 130 kD glycoform is thought to be activation-associated form primarily expressed on myeloid cells, pre-B cells, and activated T cells. It has been reported that CD43 binds to CD54 and Siglec-1. CD43 plays dual roles in cell adhesion and anti-adhesion, as well as costimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors. The 1B11 antibody reacts with the activation-associated glycoform of CD43. The epitope recognized by 1B11 is also shared with desialylated CD45RB. This antibody is useful for differentiation of effector CD8 T cells and memory T cells.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse WEHI 274.3 myeloid tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1,2, and immunohistochemistry of acetone-fixed frozen sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Jones AT, et al. 1994. J. Immunol. 153:3426. (IP, WB)
  2. Carlow DA, et al. 1999. J. Immunol. 163:1441. (IP, WB)
  3. Onami TM, et al. 2002. J. Immunol. 168:6022.
  4. van der Most RG, et al. 2003. Intl. Immunol. 15:119.
  5. Chu VT, et al. 2007. J. Immunol. 179:5947.
  6. Lang A, et al. 2008. J. Immunol. 180:4848. PubMed
  7. Gibbert K, et al. 2010. J. Immunol. 185:6179. PubMed
Product Citations
  1. Teo RYW, et al. 2018. Nat Commun. 1735:9. PubMed
  2. Venosa A, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 309: L1487 - L1498. PubMed
  3. Philip E Boulais et al. 2018. Immunity. 49(4):627-639 . PubMed
  4. Asada N, et al. 2017. Nat Cell Biol. 19:214-223. PubMed
RRID
AB_528804 (BioLegend Cat. No. 121212)

Antigen Details

Structure
Single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites, 115 kD and 130 kD glycoforms with different glycosylation and sialylation
Distribution

Most hematopoietic cells including peripheral T cells, NK cells, granulocytes, monocytes, thymocytes, platelets, plasma cells, CD5+ B-1 cells and activated B cells, and hemotopoietic progenitor cells.

Function
Play a dual roles in cell-cell adhesion and anti-adhesion, costimulation of cell activation and survival, induce apoptosis of T cells and hematopoietic progenitors
Ligand/Receptor
CD54, Siglec-1
Cell Type
B cells, Granulocytes, Hematopoietic stem and progenitors, Monocytes, NK cells, Plasma cells, Platelets, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. van den Berg TK, et al. 2001. J. Immunol. 166:3637.
2. Moore T, et al. 1994. J. Immunol. 153:4978.
3. Onami TM, et al. 2002. J. Immunol. 168:6022.
4. Tong J, et al. 2004. J. Exp. Med. 199:1277.
5. Jones AT, et al. 1994. J. Immunol. 153:3426.

Gene ID
20737 View all products for this Gene ID
UniProt
View information about CD43 on UniProt.org
Go To Top Version: 2    Revision Date: 06/27/2014

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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