Alexa Fluor® 647 anti-mouse CD98 (4F2) Antibody

Pricing & Availability
Clone
RL388 (See other available formats)
Regulatory Status
RUO
Other Names
4F2, Ly10,
Isotype
Rat IgG2a, κ
RL388_A647_042909.jpg
C57BL/6 splenocytes stained with RL388 Alexa Fluor® 647
  • RL388_A647_042909.jpg
    C57BL/6 splenocytes stained with RL388 Alexa Fluor® 647
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability
128210 100 µg $265.00
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Description

CD98 is a cell surface glycoprotein of 125 kd consisting of an 85 kd heavy chain and a 40 kd light chain.It is expressed on T, B, NK cells, granulocytes and many cell lines and tumor cells.CD98 has been shown to be involved in sodium-dependent calcium flux in muscles cells and lymphocyte activation.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Subline of mouse T lymphoma EL4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Luscher B, et al. 1985. J. Immunol. 135 :3951
  2. MacDonald HR, et al. 1985. J. Immunol. 135 :3944
Product Citations
  1. Cibrián D, et al. 2020. J Allergy Clin Immunol. 145:199. PubMed
  2. Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed
  3. Low MSY, et al. 2019. Cell Reports. 29(9):2634-2645.e5. PubMed
  4. Kurniawan H, et al. 2020. Cell Metabolism. 31(5):920-936. PubMed
  5. Gabriel SS, et al. 2021. Immunity. 54(8):1698-1714.e5. PubMed
  6. Cibrian D, et al. 2016. Nat Immunol. 17: 985-996. PubMed
  7. Trindade BC, et al. 2021. Immunity. 54:2273. PubMed
RRID
AB_2254922 (BioLegend Cat. No. 128210)

Antigen Details

Structure
Cell surface glycoprotein of 125 kd consisting of an 85 Kd heavy chain and a 40 kd light chain.
Distribution

Expressed on T cells, B cells , NK cells, granulocytes, etc.

Function
Regulatory role in amino acid transport system. Modulate sodium-dependent calcium flux and cell activation
Cell Type
B cells, Granulocytes, NK cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Luscher B, et al. 1985. J. Immunol. 135 :3951
2. MacDonald HR, et al. 1985. J. Immunol. 135 :3944

Gene ID
17254 View all products for this Gene ID
UniProt
View information about CD98 on UniProt.org
Go To Top Version: 2    Revision Date: 06/27/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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