- Clone
- QA20A67 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2
- Isotype
- Rat IgG1, κ
-
C57BL/6 mouse splenocytes were surfaced stained with anti-mouse CD4 (clone GK1.5) PE. Cells were fixed and permeabilized with True Nuclea™ Transcription Factor Buffer Set (Cat. No. 424401), and intracellularly stained with anti-mouse FOXP3 recombinant (clone QA20A67) Alexa Fluor® 647 (left) or rat IgG1, κ Alexa Fluor® 647 isotype control (right).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 118905 | 25 µg | $195.00 | |||
| 118906 | 100 µg | $375.00 | |||
FOXP3, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX, is a 47 kD transcription factor. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Recombinant
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Clone QA20A67 can completely block the binding of clone MF-14 on target cells.
- RRID
-
AB_3097539 (BioLegend Cat. No. 118905)
AB_3097539 (BioLegend Cat. No. 118906)
Antigen Details
- Structure
- Forkhead/winged-helix transcription factor
- Distribution
-
T regulatory cells
- Function
- Transcription Factor
- Cell Type
- Lymphocytes, T cells, Tregs
- Biology Area
- Immunology, Transcription Factors
- Antigen References
-
- Ono M, et al. 2007. Nature. 446:685-9.
- Hori S, et al. 2003. Science. 299:1057-61.
- Fontenot JD, et al. 2003. Nat Immunol. 4:330-6.
- Gene ID
- 20371 View all products for this Gene ID
- UniProt
- View information about FOXP3 on UniProt.org
Other Formats
View All FOXP3 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-mouse FOXP3 Recombinant Antibody | QA20A67 | ICFC |
| Purified anti-mouse FOXP3 Recombinant Antibody | QA20A67 | ICFC |
| Alexa Fluor® 647 anti-mouse FOXP3 Recombinant Antibody | QA20A67 | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-mouse FOXP3 Recombinant Antibody
C57BL/6 mouse splenocytes were surface stained with anti-mou... -
Purified anti-mouse FOXP3 Recombinant Antibody
C57BL/6 mouse splenocytes were surface stained with anti-mou... -
Alexa Fluor® 647 anti-mouse FOXP3 Recombinant Antibody
C57BL/6 mouse splenocytes were surfaced stained with anti-mo...