Alexa Fluor® 647 anti-mouse H-2Dd Antibody

Pricing & Availability
Clone
34-2-12 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class I
Isotype
Mouse (C3H/HeJ) IgG2a, κ
34-2-12_Alx647_011408
BALB/c mouse splenocytes stained with 34-2-12 Alexa Fluor® 647
  • 34-2-12_Alx647_011408
    BALB/c mouse splenocytes stained with 34-2-12 Alexa Fluor® 647
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability
110612 100 µg $229.00
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Description

The 34-2-12 antibody recognizes α3 domain of H-2Dd MHC class I alloantigen expressed on nucleated cells from mice of the H-2Dd haplotype. H-2Dd is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 34-2-12 antibody cross-reacts with cells from the C3H.LG/Ckc strain. Reactivity with other haplotypes (e.g., b,f,k,p,q,r,s) has not been reported. Clone 34-2-12 recognizes the α3 domain of Dd.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
(C57BL/6 x DBA/2)F1 mouse splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity1, immunoprecipitation2,3, blocking of cell adhesion4,5, and immunohistochemical staining of acetone-fixed frozen sections6.

This clone does not cross react with other haplotypes (e.g., b, f, k, p, q, r, s).

Application References

(PubMed link indicates BioLegend citation)
  1. Ozato K, et al. 1982. Transplantation 34:113.
  2. McCluskey J, et al. 1985. Cell 40:247.
  3. McCluskey J, et al. 1986. J. Immunol. 136:1472.
  4. Kane KP. 1994. J. Exp. Med. 179:1011.
  5. Daniels BF, et al. 1994. J. Exp. Med. 180:687.
  6. Nikolic B, et al. 1999. J. Immunol. 162:3402.
  7. Ma XT, et al. 2006. Cancer Research 66:1169.
  8. Matsumoto N, et al. 1998. Immunity 8:245.
Product Citations
  1. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  2. Parikh BA, et al. 2020. Cell Rep. 32:107969. PubMed
  3. Sivori S, et al. 2020. Cell Reports. 16(5):430-441. PubMed
RRID
AB_492913 (BioLegend Cat. No. 110612)

Antigen Details

Structure
MHC class I
Distribution

Nucleated cells of the H-2Dd haplotype

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD8
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.

Gene ID
14964 View all products for this Gene ID
UniProt
View information about H-2Dd on UniProt.org
Go To Top Version: 2    Revision Date: 06/27/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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