Alexa Fluor® 647 anti-mouse Perforin Antibody

Pricing & Availability
Clone
S16009A (See other available formats)
Regulatory Status
RUO
Other Names
Prf1, Pfp, Pfn, Pfn-1
Isotype
Rat IgG2a, κ
S16009A_AF647_Perforin_Antibody_051122
C57 splenocytes were stained with anti-mouse CD49b PE, fixed and permeabilized, then intracellularly stained with anti-mouse Perforin (clone S16009A) Alexa Fluor® 647 (left) or rat IgG2a, κ Alexa Fluor® 647 isotype control (right).
  • S16009A_AF647_Perforin_Antibody_051122
    C57 splenocytes were stained with anti-mouse CD49b PE, fixed and permeabilized, then intracellularly stained with anti-mouse Perforin (clone S16009A) Alexa Fluor® 647 (left) or rat IgG2a, κ Alexa Fluor® 647 isotype control (right).
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability
154307 25 µg $153.00
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154308 100 µg $375.00
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Description

Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse perforin transfected cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Clone S16009A reacts with perforin from both C57BL/6 and BALB/c strains.

RRID
AB_2922480 (BioLegend Cat. No. 154307)
AB_2922480 (BioLegend Cat. No. 154308)

Antigen Details

Structure
70 kD Protein that oligomerizes for pore formation.
Distribution

CTL, NK (Cytoplasmic Granules)

Function
Target cell lysis
Cell Type
NK cells, T cells
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Podack ER, et al. 1988. Immunol. Rev. 103:203.
  2. Konjar S, et al. 2010. Immunology. 131:257.
  3. Trapani JA, et al. 2002. Nat. Rev. Immunol. 2:735.
  4. Osinska I, et al. 2014. Cent. Eur. J. Immnol. 39:109.
Gene ID
18646 View all products for this Gene ID
UniProt
View information about Perforin on UniProt.org
Go To Top Version: 1    Revision Date: 05/11/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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