Alexa Fluor® 647 anti-mouse TCR Vβ8.1, 8.2 Antibody

Pricing & Availability
Clone
KJ16-133.18 (See other available formats)
Regulatory Status
RUO
Other Names
T cell receptor Vβ8.1, 8.2 chain, TCR Vβ8.1, 8.2
Isotype
Rat IgG2a, κ
KJ16-133dot18_Alx647_090208.jpg
BALB/c splenocytes stained with KJ16-133,18 Alexa Fluor® 647
  • KJ16-133dot18_Alx647_090208.jpg
    BALB/c splenocytes stained with KJ16-133,18 Alexa Fluor® 647
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability
118414 100 µg $241.00
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Description

The TCR Vβ 8 family consists of three members, namely Vβ8.1, 8.2 and 8.3. KJ16-133.18 reacts with Vβ8.1, 8.2 only, but not Vβ8.3. Most mouse strains have about 20% Vβ 8+ T cells in the periphery.However, C57BR, C57L, SJL, SWR (Tcra haplotype) and RIII strains (Tcrc haplotype) do not express Vβ8 due to a gene deletion at the Vβ 8 family loci.Other strains expressing endogenous super-antigen, such as Mls-1a have significant reduced number of Vβ 8.1 cells in the periphery.Exogenous superantigen, such as staphylococcal enterotoxin B, stimulates lymphocytes bearing Vβ 8 and selectively eliminate those T cells in vivo.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
TCR receptor from T cell hybridoma DO-11.10
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Reider SA, et al. 2011. Infect Immun. 79:3141. PubMed.
Product Citations
  1. Dikiy S, et al. 2021. Immunity. 54(5):931-946.e11. PubMed
  2. Waight JD, et al. 2018. Cancer Cell. 33:1033. PubMed
RRID
AB_1186096 (BioLegend Cat. No. 118414)

Antigen Details

Structure
TCR is a 85-kd hetero-dimer composed of alpha and beta chain. The mouse Vβ8 family has three members, namely Vβ8.1, 8.2 and 8.3.
Distribution

Expressed on α:β T cells in mouse thymus, spleen and other lymphoid organs.

Function
Recognition of peptide/ MHC
Interaction
CD3, CD4, CD8
Cell Type
T cells
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References

1. Haskins K, et al. 1984. J. Exp. Med. 160:452
2. Behlke MA, et al. 1986. P. Natl. Acad. Sci. USA 83:767
3. Fairchild S, et al. 1992. 36:189
4. White J, et al. 1989. Cell 56:27

Gene ID
21577 View all products for this Gene ID
UniProt
View information about TCR Vbeta8.1 8.2 on UniProt.org
Go To Top Version: 2    Revision Date: 06/27/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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