Alexa Fluor® 647 anti-XBP-1s Antibody

Pricing & Availability
Clone
143F (See other available formats)
Regulatory Status
RUO
Other Names
X-box binding protein 1
Isotype
Mouse IgG2a, κ
143F_AF647_Xbp-1s_Antibody_FC_1_061714
Human hepatocellular carcinoma cell line, HEPG2, was incubated overnight with (top) or without (bottom) thapsigargin (300 nM). Cells were then treated with FOXP3 Fix/Perm Buffer Set, and then stained with anti-Human Xbp-1s (clone 143F) Alexa Fluor® 647 (filled histogram) or mouse IgG2a, κ Alexa Fluor® 647 isotype control (open histogram).
  • 143F_AF647_Xbp-1s_Antibody_FC_1_061714
    Human hepatocellular carcinoma cell line, HEPG2, was incubated overnight with (top) or without (bottom) thapsigargin (300 nM). Cells were then treated with FOXP3 Fix/Perm Buffer Set, and then stained with anti-Human Xbp-1s (clone 143F) Alexa Fluor® 647 (filled histogram) or mouse IgG2a, κ Alexa Fluor® 647 isotype control (open histogram).
  • 143F_AF647_Xbp-1s_Antibody_FC_2_061714
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Cat # Size Price Quantity Check Availability
647505 25 tests $159.00
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647506 100 tests $382.00
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Description

XBP-1 is a transcription factor containing a bZIP domain. It was first identified because of its ability to bind X-box, a conserved transcriptional element in the promoter of human HLA DR gene. XBP-1 has multiple functions. It controls MHC class II gene regulation and is also essential for differentiation of plasma cells. XBP-1 upregulates as part of the ER stress response, also known as the unfolded protein response. Unspliced XBP-1 is 261 amino acids and migrates on the SDS-PAGE around 33 kD; spliced XBP-1, recognized by clone 143F, is 371 amino acids and migrates around 55 kD.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
XBP-1s recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence14 and immunohistochemical staining of PFA-fixed paraffin-embedded sections1,14.

Application References

(PubMed link indicates BioLegend citation)
  1. Tsang KY, et al. 2007. PLOS Biol. 5:568. (IHC) PubMed
  2. Farhan H, et al. 2008. EMBO J. 27:2043 (WB) PubMed
  3. Farhan H, et al. 2010. J. Cell Biol. 189:997. PubMed
  4. Feng-Jin G, et al. 2010. Cell Signal. [Epub ahead of print]
  5. Wang L, et al. 2011. Hum Mol Genet. PubMed
  6. Li J, et al. 2011. J. Biol Chem. 286:4912. PubMed
  7. Wang L, et al. 2011. Hum Mol Genet. 20:1008. PubMed
  8. Liu Y, et al. 2011. J Biol Chem. 286:13161. PubMed
  9. Shirley CM, et al. 2011 Blood. 117:6297. PubMed
  10. Vidal RL., et al. 2012. Hum Mol Genet. PubMed
  11. Byrd AE, et al. 2012. J Cell Biol. 196:689. PubMed
  12. Dickie LJ, et al. 2012. Ann rheum Dis. 71:2035. PubMed
  13. Bonetti P, et al. 2013. Blood. 122:2233. PubMed
  14. Maestre L, et al. 2009. Haematologica. 94:419. (IF, IHC)
  15. Rodriguez M, et al. 2014. J Biol Chem. 289:22942. PubMed
Product Citations
  1. Shi X, et al. 2022. NPJ Precis Oncol. 6:58. PubMed
RRID
AB_2563449 (BioLegend Cat. No. 647505)
AB_2563449 (BioLegend Cat. No. 647506)

Antigen Details

Distribution

Ubiquitously expressed.

Function
Upregulated by ER stress, IL-6, and IL-4. Downregulation correlates with tumor progression in prostate cancer. Downregulated by PAX5 transcription factor.
Biology Area
Cell Biology
Antigen References

1. Liou HC, et al. 1990. Science 247:1581.
2. Ponath PD, et al. 1993. J. Biol. Chem.268:17074.
3. Takahashi S, et al. 2002. Prostate 50:154.

Gene ID
7494 View all products for this Gene ID
UniProt
View information about XBP-1s on UniProt.org
Go To Top Version: 4    Revision Date: 06/15/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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