Alexa Fluor® 700 anti-human CD4 Antibody

Pricing & Availability
Clone
A161A1 (See other available formats)
Regulatory Status
RUO
Other Names
T4/Leu-3
Isotype
Rat IgG2b, κ
A161A1_A700_CD4_Antibody_052716
Human peripheral blood lymphocytes were stained with CD4 (clone A161A1) Alexa Fluor® 700 (filled histogram) or rat IgG2b, κ Alexa Fluor® 700 isotype control (open histogram).
  • A161A1_A700_CD4_Antibody_052716
    Human peripheral blood lymphocytes were stained with CD4 (clone A161A1) Alexa Fluor® 700 (filled histogram) or rat IgG2b, κ Alexa Fluor® 700 isotype control (open histogram).
Compare all formats See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Check Availability
357417 25 tests $136.00
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357418 100 tests $265.00
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Description

CD4, also known as T4/Leu-3, is a 55 kD single-chain type I transmembrane glycoprotein and member of the immunoglobulin superfamily. It is expressed on most thymocytes, helper T cells, type II NKT cells, and monocytes/macrophages. CD4 is part of the TCR/CD3 complex, binds to β2 domain from the MHC class II molecule, and participates in TCR signal transduction. CD4 is the receptor of IL-16 and is a coreceptor for the human immunodeficiency virus (HIV) and human herpes virus 7 (HHV-7).

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human CD4 T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Product Citations
  1. Chow A 2023. Immunity. 56(1):93-106.e6. PubMed
  2. Lee KM, et al. 2022. Cancer Immunol Res. 10:829. PubMed
  3. Stevenson EM, et al. 2022. Nat Commun. 13:4888. PubMed
  4. Mender I, et al. 2020. Cancer Cell. 38(3):400-411.e6. PubMed
RRID
AB_2616932 (BioLegend Cat. No. 357417)
AB_2616932 (BioLegend Cat. No. 357418)

Antigen Details

Structure
55 kD, type I transmembrane glycoprotein, member of the Ig superfamily
Distribution

Helper T cells, subset of thymocytes, monocytes/macrophages, type II NKT cells

Interaction
Part of the TCR/CD3 complex
Ligand/Receptor
β2 domain from the MHC class II
Cell Type
T cells, Monocytes, Macrophages, NKT cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Zhu J, et al. 2010. Annu Rev. Immunol. 28:445.
2. Vignali DA. 2010. J. Immunol. 184:5933.
3. Zhou L, et al. 2009. Immunity 30:646.
4. Singer A, et al. 2008. Nat. Rev. Immunol. 8:788.
5. Zhu J and Paul WE. 2008. Blood 112:1557.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org
Go To Top Version: 1    Revision Date: 05/27/2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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