Alexa Fluor® 700 anti-mouse CD45 Recombinant Antibody

Pricing & Availability
Clone
QA17A26 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5, LCA
Isotype
Mouse IgG1, κ
QA17A26_A700_CD45_Recombinant_Antibody_030821
C57BL/6 mouse splenocytes were stained with anti-mouse CD45 recombinant (clone QA17A26) Alexa Fluor 700 (filled histogram) or mouse IgG1, ĸ Alexa Fluor 700 isotype control (open histogram).
  • QA17A26_A700_CD45_Recombinant_Antibody_030821
    C57BL/6 mouse splenocytes were stained with anti-mouse CD45 recombinant (clone QA17A26) Alexa Fluor 700 (filled histogram) or mouse IgG1, ĸ Alexa Fluor 700 isotype control (open histogram).
Compare all formats See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Check Availability
157615 25 µg $101.00
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157616 100 µg $229.00
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Description

CD45 is known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes or platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

The QA17A26 antibody can block 30-F11 clone  and vice versa. The clone QA17A26 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Application References

(PubMed link indicates BioLegend citation)
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. Proc. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
RRID
AB_2890719 (BioLegend Cat. No. 157615)
AB_2890719 (BioLegend Cat. No. 157616)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

Expressed on almost all hematopoietic cells except for mature erythrocytes.

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References
  1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
  2. Trowbridge IS and Thomas ML. 1994. Annu. Rev. Immunol. 12:85-116.
  3. Kishihara K, et al. 1993. Cell 74:143.
  4. Pulido R, et al. 1988. J. Immunol. 140:3851.

 

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org
Go To Top Version: 1    Revision Date: 03/08/2021

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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