Alexa Fluor® 700 anti-mouse IL-12/IL-23 p40 Antibody

Pricing & Availability
Clone
C15.6 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-12 p40, Interleukin-23 p40, Cytotoxic lymphocyte maturation factor (CLMF), Natural killer cell stimulatory factor (NKSF), CTL maturation factor (TcMF), T-cell stimulating factor (TSF)
Isotype
Rat IgG1, κ
C15dot6_A700_IL-12slashIL-23-p40_Antibody_072321.png
IFNγ-stimulated (2 hours) Balb/c macrophages in the presence of LPS and monensin (24 hours) were surface stained with CD11b APC and then fixed, permeabilized, and intracellularly stained with IL-12/IL-23 p40 (clone C15.6) Alexa Fluor® 700 (left) or rat IgG1, κ Alexa Fluor® 700 (right).
  • C15dot6_A700_IL-12slashIL-23-p40_Antibody_072321.png
    IFNγ-stimulated (2 hours) Balb/c macrophages in the presence of LPS and monensin (24 hours) were surface stained with CD11b APC and then fixed, permeabilized, and intracellularly stained with IL-12/IL-23 p40 (clone C15.6) Alexa Fluor® 700 (left) or rat IgG1, κ Alexa Fluor® 700 (right).
Compare all formats See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Check Availability
505213 25 µg $153.00
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505214 100 µg $393.00
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Description

The C15.6 antibody reacts with mouse IL-12 p40 subunit of the IL-12 p70 and IL-23 p40 subunit of the IL-23 p19/p40, as well as p40 monomer and homodimer, or heterodimer. The C15.6 antibody can not neutralize the bioactivity of natural or recombinant IL-12.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CHO-expressed, recombinant mouse IL-12 p70
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

ELISA or ELISPOT Capture1,2,4,6,8,10: The purified C15.6 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated C17.8 (Cat. No. 505302) antibody as the detecting antibody. This assay detects p40 as monomer, homodimer, or heterodimer complexed with p35. The LEAF™ purified antibody is suggested for ELISPOT capture.
Additional reported applications (for the relevant formats) include: immunohistochemical staining9 of paraformaldehyde-fixed saponin-treated frozen tissue sections, immunoprecipitation3, and Western blotting3.
Note: For testing mouse IL-12 p40 (monomer, dimer, heteromer) in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 431601 to 431606) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Kitagaki K, et al. 2002. Clin. Diagn. Lab Immunol. 9:1260.
  2. Reichmann G, et al. 1999. J. Immunol. 163:3354.
  3. Wysocka M, et al. 1995. Eur. J. Immunol. 25:672.
  4. Gately M. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.16.
  5. Macatonia S, et al. 1995. J. Immunol. 154:5071.
  6. O'Connell PJ, et al. 2006. Blood 107:1010.
  7. Akilov OE, et al. 2007. J. Leukocyte Biol. 2007;10.1189/jlb.0706439.
  8. Dzhagalov I, et al. 2007. J. Immunol. 178:2113.
  9. Huang LY, et al. 2001. J. Immunol. 167:1423.
  10. Xu G, et al. 2007. J. Immunol. 179:5358. PubMed
  11. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  12. Malu DT, et al. 2011. J. Immunol. 186:6271. PubMed
RRID
AB_2894642 (BioLegend Cat. No. 505213)
AB_2894642 (BioLegend Cat. No. 505214)

Antigen Details

Structure
Cytokine; monomer, heterodimer (p40:p35 or p40:p19) or homodimer (p40:p40)
Bioactivity
IL-12 p70 (p40:p35) induces IFN-γ, TNF-a production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of TH1 T lymphocytes. IL-23 (p40:p19) induces proliferation and production of IFN-γ by human me
Cell Sources
Dendritic cells, monocytes/macrophages, B cells, T cells
Cell Targets
T cells, NK cells
Receptors
IL-12Rβ1 binds p40; dimeric with IL-12Rβ2 binds p35
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Quesniaux V. 1992. Research Immunol. 143:385.
3. Trinchieri G, et al. 1992 Prog. Growth Factor Res. 4:355.
4. Trinchieri G, et al. 1993 Immunol. Today. 14:335.
5. Oppmann B, et al. 2000 Immunity. 13:715.
6. Aggarwal S, et al. 2003 J. Biol. Chem. 278:1910.
7. Parham C, et al. 2002 J. Immunol. 168:5699.
8. Belladonna ML, et al. 2002 J. Immunol. 168:5448.
9. Lankford, CS, et al. 2003 J. Leukocyte Biol. 73:49.

Regulation
Downregulated by IL-10; homodimeric p40 antagonistic to functional p70 heterodimer; p40 monomer has no function; p40 subunit in common with IL-23
Gene ID
16160 View all products for this Gene ID
UniProt
View information about IL-12/IL-23 p40 on UniProt.org
Go To Top Version: 2    Revision Date: 09/20/2023

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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