- Clone
- 17C7 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- EL2, SPTA1, Spectrin alpha chain, erythrocyte, alpha-I spectrin, erythroid alpha-spectrin
- Previously
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Covance Catalog# SIG-39700
Signet Catalog# 9700-02
Signet Catalog# 9700-05
Signet Catalog# 9700-10
- Isotype
- Mouse IgG1, κ
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Western blot of anti-Alpha-I Spectrin antibody (clone 17C7). Lane 1: Molecular weight marker; Lane 2: 50 µg of human lung lysate; Lane 3: 50 µg of mouse lung lysate; Lane 4: 50 µg of rat brain lung lysate; Lane 5: 50 µg of human kidney lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 803101 | 200 µL | $581.00 | |||
Spectrin is an intracellular cytoskeletal protein that provides structure to eukaryotic cells and helps maintain plasma membrane integrity. Alpha-1 Spectrin, encoded by the SPTA1 gene, is found primarily in erythrocytes and in the cerebellum.
Product Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant α spectrin SH3 domain
- Formulation
- Phosphate-buffered solution (no preservatives or carrier proteins).
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/mL
- Storage & Handling
- This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
- Application
-
WB - Quality tested
ICC - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody is effective in Western Blotting (WB). Additional reported applications include immunocytochemistry1, 2.
Clone 17C7 is a specific marker for human, rat and mouse alpha I spectrin. It reacts with erythroid spectrin in red blood cells and in cerebellum. It does not crossreact with alpha II (nonerythroid) spectrin SH3 domain or recombinant human fodrin SH3 domain. In cerebellar cell cultures, clone 17C7 labels cell bodies consistent with localization of erythroid spectrin. In NIH 3T3 cells, clone 17C7 reacts with a 280 kDa protein which is a candidate marker of endocytic vesicles called macropinosomes1, 2.
This antibody was developed against a recombinant alpha I spectrin (erythroid spectrin) SH3 domain. -
Application References
(PubMed link indicates BioLegend citation) -
- Xu J, et al. 2001. Brain Res. 898(1):171-7. (WB, ICC)
- Xu J, et al. 2000. J. Cell Sci.113:3805-14. (WB, ICC)
- Product Citations
-
- RRID
-
AB_2564659 (BioLegend Cat. No. 803101)
Antigen Details
- Structure
- Expected MW: 250 kD
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Presynaptic proteins, Scaffold Proteins
- Antigen References
-
- De Matteis MA, Morrow JS. 2000. J. Cell Sci. 113:2331-43.
- Bennett V, Gilligan DM. 1993. Annu. Rev. Cell Biol. 9:27-66.
- Gene ID
- 6708 View all products for this Gene ID 20739 View all products for this Gene ID 289257 View all products for this Gene ID
- UniProt
- View information about alpha-I Spectrin on UniProt.org
Other Formats
View All α-I Spectrin Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Anti-Alpha-I Spectrin | 17C7 | WB,ICC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Anti-Alpha-I Spectrin
Western blot of anti-Alpha-I Spectrin antibody (clone 17C7)....