- Clone
- 17-206 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- BG4
- Previously
-
Signet Catalog# 313-01
Covance Catalog# SIG-3313
- Isotype
- Mouse IgG3, κ
Cat # | Size | Price | Quantity Check Availability | ||
---|---|---|---|---|---|
922102 | 1 mL | $402.00 |
BG-4 is also known as blood group antigen H (type 1). The H antigen is a precursor to A and B antigens in the ABO system. The H antigen is a carbohydrate sequence consisting of a chain of β-D-galactose, β-D-N-acetylglucosamine, β-D-galactose, and 2-linked, α-L-fucose, which is linked to protein or ceramide. The H gene locus encodes the fucosyltransferase 1 protein, which is responsible for linking the carbohydrate antigen to the protein. The H antigen is expressed on erythrocytes and epithelial cells.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This antibody was developed against the SW-403 colon cancer cell line. It is specific for Blood group H (type 1).
- Formulation
-
Phosphate-buffered solution with BSA + 0.09% NaN3.
Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service. - Preparation
- Ammonium sulfate precipitated TCS.
- Storage & Handling
- Store between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunohistochemical staining.
The optimal working dilution should be determined for each specific assay condition.
• IHC: ≥1:40 for Biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501).
Tissue Sections: Formalin-fixed, paraffin-embedded tissues.
Pretreatment: Not required
Incubation: 20 minutes at room temperature - Application Notes
-
This antibody is effective in immunohistochemistry (IHC).
Positive tissue (human): Antrum. -
Application References
(PubMed link indicates BioLegend citation) -
- Lloyd KO, et al. 1983.Immunogen. 17:537.
- Sakamoto J, et al. 1984. Mol Immunol. 21:1093.
- Anger BR, et al. 1982. Hybridoma. 1:139.
- Product Citations
-
- RRID
-
AB_2565409 (BioLegend Cat. No. 922102)
Antigen Details
- Biology Area
- Cell Biology
- Molecular Family
- Blood Group Markers
- Gene ID
- NA
- UniProt
- View information about BG-4 on UniProt.org
Other Formats
View All BG-4 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Anti-BG-4 | 17-206 | IHC-P |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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