- Clone
- 185D (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Silent information regulator 3, CMT1, MAR2, STE8
- Previously
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NeoClone Catalog# W0036
- Isotype
- Mouse IgG1
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Western blot analysis of 50 ng of recombinant yeast Sir3 (lane 1), 15 µg E. coli BL21 (lane 2), yeast (saccharomyces cerevisiae) (lane 3) and HeLa (lane 4) using anti-yeast Sir3 (clone 185D) antibody followed by HRP goat anti-mouse IgG antibody.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 669103 | 100 µL | $303.00 | |||
SIR1, SIR2, SIR3, and SIR4 are proteins that are required for transcriptional repression of the silent mating type loci, HML and HMR. SIR2, SIR3, and SIR4 proteins repress multiple loci by modulating chromatin structure, which involves compaction of chromatin fiber into a more condensed form.
Product Details
- Verified Reactivity
- Yeast
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant protein
- Preparation
- Dried ascites
- Storage & Handling
- Store lyophilized antibody at -20°C.
- Application
-
WB - Quality tested
ELISA - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. To reconstitute, add 100 µl of ddH2O to the tube containing antibody and mix to dissolve. For Western blotting, the suggested dilution is 1:1000 after reconstitution. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Previously item# W0036 from Neoclone.
- RRID
-
AB_2564540 (BioLegend Cat. No. 669103)
Antigen Details
- Structure
- Sir3 is a 111 kD Saccharomyces cervisiae transcription repressor and is a member of the Sir (Silent Information Regulator) family.
- Biology Area
- Cell Biology, Transcription Factors
- Gene ID
- 851163 View all products for this Gene ID
- UniProt
- View information about Sir3 on UniProt.org
Other Formats
View All Sir3 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Anti-yeast Sir3 | 185D | WB,ELISA |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Anti-yeast Sir3
Western blot analysis of 50 ng of recombinant yeast Sir3 (la...