APC anti-human CD11a/CD18 (LFA-1) Antibody

Pricing & Availability
Clone
m24 (See other available formats)
Regulatory Status
RUO
Other Names
Integrin β2 subunit, LFA -1 β subunit, β2 integrin, b2 subunit, LFA-1b, LFA-1 beta, LFA1b, LFA1 beta, beta 2 integrin subunit LFA
Isotype
Mouse IgG1, κ
m24_APC_CD11aCD18_Antibody_091517
PMA-stimulated human peripheral blood granulocytes were stained with CD11/CD18 (clone m24) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
  • m24_APC_CD11aCD18_Antibody_091517
    PMA-stimulated human peripheral blood granulocytes were stained with CD11/CD18 (clone m24) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
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363409 25 tests $113.00
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363410 100 tests $291.00
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Description

CD11/CD18 belongs to the integrin family of proteins. It is heterodimeric cell surface receptor expressed on all leukocytes. CD18, in association with integrin α chain CD11a, CD11b, and CD11c forms LFA-1, Mac-1, and αXβ2, respectively, and plays an important role in leukocyte adhesion. CD11/CD18 complexes bind ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, and fibrinogen. Clone m24 binds the extended/open high affinity conformation of CD11a/CD18. The antibody can be used as a reporter of the activation state of the integrin receptor in response to exposure to Mg2+ or Mn2+.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Fibronectin-purified human monocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone m24 can be used as a reporter of the activation state of integrin receptor in response to exposure with Mg2+ or Mn2+.

Application References

(PubMed link indicates BioLegend citation)
  1. Hogg N. et al. 1985. Cell Immunol. 92:247.
  2. McDowall A. et al. 2003. J. Clin. Invest. 111:51.
Product Citations
  1. Zhang W, et al. 2022. Dev Cell. 57:329. PubMed
  2. Hamdi L, et al. 2022. Ann Clin Transl Neurol. 9:1792. PubMed
  3. Bouafia A, et al. 2018. J Clin Invest. 129:1047. PubMed
  4. Wu X, et al. 2021. Front Immunol. 12:731767. PubMed
  5. Hegewisch-Solloa E, et al. 2021. J Immunol. 207:950. PubMed
  6. Kolchakova D, et al. 2021. Biomolecules. 11:. PubMed
  7. Lee CH, et al. 2018. Elife. 7:e32532. PubMed
  8. Högelin KA, et al. 2021. iScience. 24:103078. PubMed
RRID
AB_2716070 (BioLegend Cat. No. 363409)
AB_2716070 (BioLegend Cat. No. 363410)

Antigen Details

Structure
Integrin family, 95KD, Ig superfamily
Distribution

All leukocytes

Function
Adhesion
Ligand/Receptor
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, fibrinogen
Cell Type
Dendritic cells, Leukocytes, Neutrophils
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Anderson D, et al. 1987. Annu. Rev. Med. 38:175.
2. Springer T. 1994. Cell 76:301.

Gene ID
16408 View all products for this Gene ID 16414 View all products for this Gene ID
UniProt
View information about CD18 on UniProt.org
Go To Top Version: 0    Revision Date: 09/18/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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