- Clone
- QA20A58 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- alpha-chain of C3bi receptor, alphaM, C3bi receptor, CD11b, gp155/95, integrin a, M-chain, Mac-1
- Isotype
- Mouse IgG2a, κ
-
Human lysed whole blood (monocyte population) was stained with anti-human CD14 Pacific Blue™ and anti-human CD11b recombinant (clone QA20A58) APC (left) or mouse IgG2a, κ APC isotype control (right).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 379905 | 25 tests | $100.00 | |||
| 379906 | 100 tests | $340.00 | |||
CD11b (integrin alpha M subunit) is a type I transmembrane glycoprotein. CD11b combines with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin alpha M/beta 2, also known as Mac-1 and complement receptor type 3 (CR3). Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen. CD11b is primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
The binding of clone QA20A58 can be completely blocked by reference clone.
Other anti-hCD11b clone ICRF44 does not affect the binding of clone QA20A58 to target. - RRID
-
AB_3068038 (BioLegend Cat. No. 379905)
AB_3068038 (BioLegend Cat. No. 379906)
Antigen Details
- Structure
- Integrin family, associates with integrin β2 (CD18), 170 kD
- Distribution
-
Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subsets of T and B cells
- Function
- Adhesion, chemotaxis, phagocytosis, neutrophil activation
- Ligand/Receptor
- ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, fibrinogen
- Cell Type
- B cells, Dendritic cells, Granulocytes, Neutrophils, NK cells, T cells, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
- Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
- Springer TA. 1994. Cell. 76:301-14.
- Coxon A, et al. 1996. Immunity. 5:653-66.
- Gene ID
- 3684 View all products for this Gene ID
- UniProt
- View information about CD11b on UniProt.org
Other Formats
View All CD11b Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-human CD11b Recombinant Antibody | QA20A58 | FC |
| Purified anti-human CD11b Recombinant Antibody | QA20A58 | FC |
| APC anti-human CD11b Recombinant Antibody | QA20A58 | FC |
| PE/Cyanine7 anti-human CD11b Recombinant Antibody | QA20A58 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human CD11b Recombinant Antibody
Human peripheral blood monocytes were stained with anti-huma... -
Purified anti-human CD11b Recombinant Antibody
Human peripheral blood monocytes were stained with purified ... -
APC anti-human CD11b Recombinant Antibody
Human lysed whole blood (monocyte population) was stained wi... -
PE/Cyanine7 anti-human CD11b Recombinant Antibody
Human lysed whole blood (monocyte population) was stained wi...