APC anti-human CD14 Antibody

Pricing & Availability
Clone
63D3 (See other available formats)
Regulatory Status
RUO
Other Names
Monocyte differentiation antigen CD14, myeloid cell-specific leucine-rich glycoprotein, LPS receptor
Isotype
Mouse IgG1, κ
63D3_APC_CD14_Antibody_020816
Human peripheral blood monocytes were stained with APC anti-human CD14 (clone 63D3) (filled histogram) or APC mouse IgG1, κ isotype control (open histogram).
  • 63D3_APC_CD14_Antibody_020816
    Human peripheral blood monocytes were stained with APC anti-human CD14 (clone 63D3) (filled histogram) or APC mouse IgG1, κ isotype control (open histogram).
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367117 25 tests $125.00
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367118 100 tests $267.00
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Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein that is also known as the LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokine expression in monocytes and neutrophils.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified human peripheral blood monocytes.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Fridlender ZG, et al. 1999. Hum. Immunol. 11:1028.
  2. Devitt A, et al. 1998. Nature 6675:505.
Product Citations
  1. Pan J, et al. 2021. J Cell Mol Med. 25:1089. PubMed
  2. Wheeler MM, et al. 2022. Nat Commun. 13:7592. PubMed
  3. Han CY, et al. 2022. Redox Biol. 51:102263. PubMed
  4. Aubrey BJ, et al. 2022. Nat Cancer. 3:595. PubMed
  5. Gibellini L, et al. 2020. EMBO Mol Med. 12:e13001. PubMed
  6. Linden G, et al. 2021. RNA Biol. 18:604. PubMed
  7. Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed
  8. Slingsby MHL, et al. 2021. Haematologica. Online ahead of print. PubMed
  9. Xu Y, et al. 2021. Stem Cell Res Ther. 12:112. PubMed
  10. Qi S, et al. 2021. Biol Sex Differ. 12:66. PubMed
  11. Mehta AK, et al. 2021. Nat Cancer. 2:66. PubMed
  12. Obradovic A, et al. 2021. Cell. 184(11):2988-3005.e16. PubMed
  13. Hijmans JG, et al. 2019. J Am Heart Assoc. 8:e011134. PubMed
  14. James KR, et al. 2020. Nat Immunol. 1.113194444. PubMed
  15. Wang T et al. 2018. Immunity. 49(3):504-514 . PubMed
  16. Medapati MR, et al. 2021. FASEB J. 35:e21375. PubMed
  17. Gouwy M, et al. 2016. PLoS One. 11:e0166006. PubMed
  18. Mathewson ND, et al. 2021. Cell. 184(5):1281-1298.e26. PubMed
  19. Keskin DB, et al. 2019. Nature. 565:234. PubMed
  20. Qiu X, et al. 2022. Cell. 185:690. PubMed
  21. Bailey AL, et al. 2020. bioRxiv. . PubMed
  22. de Silva S, et al. 2020. Cancer Immunol Res. 0.493055556. PubMed
  23. Slyper M, et al. 2020. Nat Med. 26:792. PubMed
RRID
AB_2566791 (BioLegend Cat. No. 367117)
AB_2566791 (BioLegend Cat. No. 367118)

Antigen Details

Structure
GPI-linked membrane glycoprotein.
Distribution

Monocytes, macrophages, dendritic cells, and granulocytes.

Function
LPS receptor, clearance of Gram-negative pathogens.
Interaction
LPS.
Ligand/Receptor
LPS.
Cell Type
Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Stocks SC, et al. 1990. Biochem. J. 268:275.
  2. Wright SD, et al. 1990. Science 4975:1431.
Gene ID
929 View all products for this Gene ID
UniProt
View information about CD14 on UniProt.org
Go To Top Version: 1    Revision Date: 02/08/2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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