APC anti-human CD14 Antibody

Pricing & Availability
Clone
M5E2 (See other available formats)
Regulatory Status
RUO
Workshop
III 329
Other Names
LPS receptor
Isotype
Mouse IgG2a, κ
M5E2
Human peripheral blood monocytes stained with M5E2 APC
  • M5E2
    Human peripheral blood monocytes stained with M5E2 APC
Compare all formats See APC spectral data
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301807 25 tests $65.00
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301808 100 tests $135.00
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Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens, and in the upregulation of adhesion molecules and expression of cytokines in monocytes and neutrophils.

Technical data sheet

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Capuchin Monkey, Cow, Chimpanzee, Common Marmoset, Cotton-topped Tamarin, Dog, Pigtailed Macaque, Squirrel Monkey
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length human CD14 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation4, and immunofluorescence microscopy5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301861 and 301862).

Application References

(PubMed link indicates BioLegend citation)
  1. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
  2. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (IHC-F)
  3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  4. Power CP, et al. 2004. J. Immunol. 173:5229. (Block)
  5. Williams KC, et al. 2001. J. Exp. Med. 193:905.
  6. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (FC) PubMed
  7. Santer DM, et al. 2010. J. Immunol. 485:4739. PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Zizzo G, et al. 2012. J. Immunol. 189:3508. PubMed
  10. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  11. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Vondra S, et al. 2023. Cell Rep. 42:111977. PubMed
  2. Fehlberg LK, et al. 2023. J Dairy Sci. :. PubMed
  3. Tang C, et al. 2023. Nat Commun. 14:1493. PubMed
  4. Zhu ZY, et al. 2022. Front Immunol. 12:781087. PubMed
  5. Lopes MG, et al. 2021. J Dairy Sci. 104:9340. PubMed
  6. Mahmud FJ, et al. 2020. J Neuroinflammation. 0.897916667. PubMed
  7. Downes DJ, et al. 2021. Nat Genet. 53:1606. PubMed
  8. Woolsey C, et al. 2020. Scientific Reports. 10(1):3071.. PubMed
  9. Alharthi AS, et al. 2019. J Dairy Sci. 102:10599. PubMed
  10. Guo Z, et al. 2022. NPJ Precis Oncol. 6:34. PubMed
  11. Ferrari M, et al. 2021. J Virol. 95:e0068521. PubMed
  12. Offersen R, et al. 2016. J Virol. 90: 4441 - 4453. PubMed
  13. Woolsey C et al. 2019. Cell Rep. 28(12):3032-3046 . PubMed
  14. Kedage V, et al. 2019. MAbs. 12:1685832. PubMed
  15. Yokoyama K, et al. 2022. Front Immunol. 13:1016914. PubMed
  16. Jankeel A, et al. 2020. J Virol. :94. PubMed
  17. Constantinescu-Bercu A, et al. 2020. eLife. 9:00. PubMed
  18. Spildrejorde M, et al. 2014. Physiol Genomics. 46:512. PubMed
  19. Mu Z, et al. 2021. Genome Biol. 122:22. PubMed
  20. Takele Y, et al. 2022. PLoS Negl Trop Dis. 16:e0010544. PubMed
  21. Woodberry T, et al. 2017. Infect Immun. 85:e00986. PubMed
  22. Claus C, et al. 2021. Pharmaceutics. 13:. PubMed
  23. Wegner J, et al. 2021. Sci Rep. 11:14983. PubMed
  24. Broeren MGA, et al. 2018. ALTEX. 36:18:00. PubMed
  25. da Rocha Sobrinho HM, et al. 2021. Journal of Neuroscience Research. 99:2511. PubMed
  26. Powell EJ, et al. 2018. J Dairy Sci. 101:8182. PubMed
  27. Ferguson Bennit HR, et al. 2017. Blood Lymphat Cancer. 7:9. PubMed
  28. Rhoades NS, et al. 2022. Cell Rep. 39:110725. PubMed
  29. Weiss R, et al. 2021. Cells. 10:. PubMed
  30. Chen Y, et al. 2021. Front Mol Biosci. 8:777370. PubMed
  31. Zhou Z, et al. 2018. J Dairy Sci. 101:10374. PubMed
  32. Tähtinen S, et al. 2022. Nat Immunol. 23:532. PubMed
  33. Kiers D et al. 2018. EBioMedicine. 33:144-156 . PubMed
  34. Lavaert M, et al. 2020. Immunity. 52(6):1088-1104. PubMed
  35. Duncan CJA, et al. 2022. J Exp Med. 219:. PubMed
  36. Bade RM, et al. 2021. Molecular Oncology. . PubMed
  37. Miksiunas R, et al. 2020. Int J Mol Sci. 21:00. PubMed
  38. Alhaj Hussen K, et al. 2020. Front Immunol. 11:579776. PubMed
  39. Wang H, et al. 2017. J Neurochem. 141:347. PubMed
  40. Thomas BL, et al. 2022. J Dairy Sci. 105:2256. PubMed
  41. Magg T, et al. 2021. Sci Immunol. 6:. PubMed
RRID
AB_314189 (BioLegend Cat. No. 301807)
AB_314189 (BioLegend Cat. No. 301808)

Antigen Details

Structure
GPI-linked membrane glycoprotein, 53-55 kD
Distribution

Monocytes, macrophages, granulocytes (low)

Function
LPS receptor, clearance of Gram-negative pathogens
Ligand/Receptor
LPS
Cell Type
Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.

Gene ID
929 View all products for this Gene ID
UniProt
View information about CD14 on UniProt.org

Other Formats

View All CD14 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD14 M5E2 FC
FITC anti-human CD14 M5E2 FC
PE anti-human CD14 M5E2 FC
Purified anti-human CD14 M5E2 FC,CyTOF®,Block,IHC-F
PE/Cyanine7 anti-human CD14 M5E2 FC
Alexa Fluor® 488 anti-human CD14 M5E2 FC
Alexa Fluor® 647 anti-human CD14 M5E2 FC
Ultra-LEAF™ Purified anti-human CD14 M5E2 FC,CyTOF®,Block,IHC-F
Pacific Blue™ anti-human CD14 M5E2 FC
APC/Cyanine7 anti-human CD14 M5E2 FC
Alexa Fluor® 700 anti-human CD14 M5E2 FC
PerCP/Cyanine5.5 anti-human CD14 M5E2 FC
Biotin anti-human CD14 M5E2 FC
Brilliant Violet 421™ anti-human CD14 M5E2 FC
Brilliant Violet 570™ anti-human CD14 M5E2 FC
Brilliant Violet 605™ anti-human CD14 M5E2 FC
Brilliant Violet 650™ anti-human CD14 M5E2 FC
Brilliant Violet 711™ anti-human CD14 M5E2 FC
Brilliant Violet 785™ anti-human CD14 M5E2 FC
Brilliant Violet 510™ anti-human CD14 M5E2 FC
Purified anti-human CD14 (Maxpar® Ready) M5E2 FC,CyTOF®
PerCP anti-human CD14 M5E2 FC
FITC anti-human CD14 M5E2 FC
PE/Dazzle™ 594 anti-human CD14 M5E2 FC
Pacific Blue™ anti-human CD14 M5E2 FC
APC/Fire™ 750 anti-human CD14 M5E2 FC
APC anti-human CD14 M5E2 FC
TotalSeq™-A0081 anti-human CD14 M5E2 PG
TotalSeq™-B0081 anti-human CD14 M5E2 PG
TotalSeq™-C0081 anti-human CD14 M5E2 PG
PE anti-human CD14 M5E2 FC
PE/Cyanine5 anti-human CD14 M5E2 FC
TotalSeq™-D0081 anti-human CD14 M5E2 PG
APC/Fire™ 750 anti-human CD14 M5E2 FC
GMP FITC anti-human CD14 M5E2 FC
PE/Cyanine7 anti-human CD14 M5E2 FC
GMP APC anti-human CD14 M5E2 FC
GMP PE anti-human CD14 M5E2 FC
PE/Dazzle™ 594 anti-human CD14 M5E2 FC
GMP Pacific Blue™ anti-human CD14 M5E2 FC
GMP APC/Fire™ 750 anti-human CD14 M5E2 FC
PerCP/Cyanine5.5 anti-human CD14 M5E2 FC
Spark Violet™ 500 anti-human CD14 M5E2 FC
GMP PE/Dazzle™ 594 anti-human CD14 M5E2 FC
APC/Fire™ 810 anti-human CD14 M5E2 FC
PE/Fire™ 700 anti-human CD14 M5E2 FC
Go To Top Version: 4    Revision Date: 11/05/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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