- Clone
- 1A5 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- VII 70443
- Other Names
- OBBP2, CD33L2, OB-BP2
- Isotype
- Mouse IgG1, κ
-
Human peripheral blood granulocytes were stained with Siglec-5 (clone 1A5) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 352005 | 25 tests | $149.00 | |||
| 352006 | 100 tests | $350.00 | |||
CD170, also known as Siglec-5, is a single pass transmembrane protein member of the immunoglobulin superfamily. On the cell surface, CD170 forms a 140 kD dimer. The cytoplasmic domain of Siglec-5 contains two ITIM motifs that recruit the tyrosine-phosphatases SHP-1 and SHP-2 after tyrosine-phosphorylation, which in turn results in the inhibition of cell signaling. Siglec-5 is expressed by granulocytes, monocytes/macrophages, subsets of lymphocytes, and a subset of activated dendritic cells. Siglec-5 binds α2,3- and α2,6-linked sialic acid as well as glycophorin A, and is involved in cell adhesion.
Product Details
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee, Bonobo, Gorilla
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Extracellular region of Siglec-5
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
The 1A5 antibody cross-reacts with Siglec-144. Additional reported applications (for the relevant formats) include: ELISA2.
Clone 1A5 cross-reacts to Gorilla2 -
Application References
(PubMed link indicates BioLegend citation) -
- Cornish AL, et al. 1998. Blood 92:2123.
- Nguyen DH, et al. 2006. P. Natl. Acad. Sci. USA 103:7765. (FC, ELISA)
- Connolly NP, et al. 2002. Br. J. Haematol. 119:221.
- Angata T, et al. 2006. FASEB J. 20:1964.
- Avril T, et al. 2005. J. Biol. Chem. 280:19843.
- Product Citations
-
- RRID
-
AB_2564262 (BioLegend Cat. No. 352005)
AB_2564262 (BioLegend Cat. No. 352006)
Antigen Details
- Structure
- Member of the immunoglobulin superfamily, single pass transmembrane protein, forms a 140 kD dimer; cytoplasmic domain contains two ITIM motifs
- Distribution
-
Granulocytes, monocytes/macrophages, subsets of lymphocytes, and activated dendritic cells
- Function
- Inhibitory receptor, cell adhesion
- Interaction
- SHP-1, SHP-2
- Ligand/Receptor
- α2,3- and α2,6-linked sialic acid, glycophorin A
- Cell Type
- B cells, Dendritic cells, Granulocytes, Lymphocytes, Macrophages, Monocytes
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules, Siglec Molecules
- Antigen References
-
1. Soto PC, et al. 2010. J. Immunol. 184:4185.
2. Carlin AF, et al. 2009. J. Exp. Med. 206:1691.
3. Zhuravleva MA. 2008. J. Mol. Biol. 375:437. - Gene ID
- 8778 View all products for this Gene ID
- UniProt
- View information about CD170 on UniProt.org
Other Formats
View All CD170 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD170 (Siglec-5) | 1A5 | FC,ELISA |
| PE anti-human CD170 (Siglec-5) | 1A5 | FC |
| APC anti-human CD170 (Siglec-5) | 1A5 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD170 (Siglec-5)
Human peripheral blood granulocytes were stained with Siglec... -
PE anti-human CD170 (Siglec-5)
Human peripheral blood granulocytes were stained with Siglec... -
APC anti-human CD170 (Siglec-5)
Human peripheral blood granulocytes were stained with Siglec...