APC anti-human CD1d Antibody

Pricing & Availability
Clone
51.1 (See other available formats)
Regulatory Status
RUO
Other Names
R3G1
Isotype
Mouse IgG2b, κ
51.1_APC_1_073012
Human peripheral blood lymphocytes were stained with CD19 FITC and CD1d (clone 51.1) APC (top) or mouse IgG2b, κ APC isotype control (bottom).
  • 51.1_APC_1_073012
    Human peripheral blood lymphocytes were stained with CD19 FITC and CD1d (clone 51.1) APC (top) or mouse IgG2b, κ APC isotype control (bottom).
  • 51.1_APC_2_073012
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350307 25 tests $137.00
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350308 100 tests $314.00
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Description

CD1d is a MHC-like, type I transmembrane protein, member of the CD1 family and the immunoglobulin superfamily. On the cell surface, CD1d forms a heterodimer with β2-microglobulin. CD1d is expressed by antigen-presenting cells such as B cells, monocytes/macrophages, dendritic cells, and some non-lymphoid cells. Cortical thymocytes express CD1d but the expression is lost in mature T cells. CD1d presents lipid antigens to iNKT cells analogous to MHC molecule presentation of peptides to T cells.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CD1d-Fc fusion
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1, Western blotting1,2, and induction of IL-12 production by crosslinking of CD1d3.

Application References

(PubMed link indicates BioLegend citation)
  1. Exley M, et al. 2000. Immunology 100:37. (IHC, WB)
  2. Durante-Mangoni E, et al. 2004. J. Immunol. 173:2159. (WB)
  3. Yue SC, et al. 2005. P. Natl. Acad. Sci. USA 102:11811. (Stim)
Product Citations
  1. Zhou Y, et al. 2021. Methods Mol Biol. 2388:35. PubMed
  2. Yamaguchi S, et al. 2021. Eur J Immunol. 51:2306. PubMed
  3. Deng C, et al. 2016. Diabetes Care. 39: 434 - 440. PubMed
  4. Deng C, et al. 2017. J Diabetes Res. 10.1155/2017/5052812. PubMed
  5. Vlugt L, et al. 2014. J Infect Dis. 210:1207. PubMed
  6. Muir LA, et al. 2022. JCI Insight. 7:. PubMed
  7. Zhu Y, et al. 2019. Cell Stem Cell. 25:542. PubMed
  8. Li YR, et al. 2021. Cell Rep Med. 2:100449. PubMed
  9. Rotolo A et al. 2018. Cancer cell. 34(4):596-610 . PubMed
RRID
AB_10642029 (BioLegend Cat. No. 350307)
AB_10642029 (BioLegend Cat. No. 350308)

Antigen Details

Structure
MHC-like, type I transmembrane protein, member of the CD1 family and the immunoglobulin superfamily
Distribution

Expressed by cortical thymocytes, monocytes, macrophages, dendritic cells, B cells (highly expressed on marginal zone B cells in particular), and in some non-lymphoid cells

Function
Present lipid antigens to iNKT cells
Ligand/Receptor
When loaded with a lipid, is recognized by the Vα14i TCR from iNKT cells
Cell Type
B cells, Dendritic cells, Macrophages, Monocytes, Thymocytes
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, MHC Antigens, TCRs
Antigen References

1. Koch M, et al. 2005. Nat. Immunol. 6:819.
2. Liu X, et al. 2010. P. Natl. Acad. Sci. USA 107:13010.
3. Zeissig S, et al. 2010. J. Clin. Invest. 120:2889.
4. Teige A, et al. 2010. J. Immunol. 185:345.

Gene ID
912 View all products for this Gene ID
UniProt
View information about CD1d on UniProt.org
Go To Top Version: 2    Revision Date: 10/04/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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