APC anti-human CD210 (IL-10 R) Antibody

Pricing & Availability
Clone
3F9 (See other available formats)
Regulatory Status
RUO
Workshop
VII 70502
Other Names
IL-10 Receptor, IL-10R
Isotype
Rat IgG2a, κ
3F9_APC_CD210_Antibody_FC_122214
Human peripheral blood lymphocytes were stained with CD210 (clone 3F9) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
  • 3F9_APC_CD210_Antibody_FC_122214
    Human peripheral blood lymphocytes were stained with CD210 (clone 3F9) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability
308811 25 tests $131.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
308812 100 tests $291.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD210, also known as the IL-10 receptor, is a 90-110 kD protein expressed on T cells, B cells, NK cells, monocytes and macrophages. CD210 belongs to the class II cytokine receptor family which includes the IFN-γ receptor (CDw119), the IFN-α/β receptor (CD118) and tissue factor (CD142). The IL-10 receptor is involved in signal transduction by inducing phosphorylation of STAT1a and STAT3 and by inducing activation of Jak1 and Tyk.

Technical data sheet

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
shIL-10R
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone 3F9 recognizes the IL-10-binding epitope of IL-10R1.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro blocking1-3 of human IL-10 binding to IL-10R. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308804) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-rat IgG second step), followed by SAv-PE (Cat. No. 405204). The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for highly sensitive assays (Cat. No. 308817 and 308818).

Application References

(PubMed link indicates BioLegend citation)
  1. Liu Y, et al. 1997. J. Immunol. 158:604. (Immunogen, IP, Block)
  2. Levings MK, et al. 2005. Blood 105:1162. (Block)
  3. Goodier MR, et al. 2000. J. Immunol. 165:139. (Block)
  4. Huang YH, et al. 2009. J. Leukoc. Biol. 86:273. PubMed
  5. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  6. Liu BS,et al. 2011. J Leukoc Biol. 89:981. PubMed
  7. Joffe M, et al. 2012. Int Immunol. 24:447. PubMed
  8. MacDonald KP, et al. 1999. J. Immunol. 163:5599. (epitope)
Product Citations
  1. Vaka R, et al. 2022. Stem Cell Res Ther. 13:20. PubMed
  2. Zhong Q, et al. 2018. J Immunol. 200:3913. PubMed
RRID
AB_2564592 (BioLegend Cat. No. 308811)
AB_2564592 (BioLegend Cat. No. 308812)

Antigen Details

Structure
Ig superfamily, class II cytokine receptor family, transmembrane glycoprotein, 90-110 kD
Distribution

T cells and B cells, NK cells, monocytes, macrophages

Function
Activates STAT1, STAT3, Jak1, Tyk
Ligand/Receptor
IL-10
Cell Type
B cells, Macrophages, Monocytes, NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Kotenko S. 2002. Cytokine Growth Factor Rev. 13:223.
2. Trinchieri G. 2003. Nat. Rev. Immunol. 3:133.

Gene ID
3587 View all products for this Gene ID
UniProt
View information about CD210 on UniProt.org
Go To Top Version: 1    Revision Date: 12/22/2014

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account