APC anti-human CD25 Antibody

Pricing & Availability
Clone
BC96 (See other available formats)
Regulatory Status
RUO
Workshop
V T-072
Other Names
Low affinity IL-2R, IL-2R α chain, Tac, p55
Isotype
Mouse IgG1, κ
BC96_APC_071805
PHA-stimulated (3 day) human peripheral blood lymphocytes were stained were with CD25 (clone BC96) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
  • BC96_APC_071805
    PHA-stimulated (3 day) human peripheral blood lymphocytes were stained were with CD25 (clone BC96) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability
302609 25 tests $44.00
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302610 100 tests $110.00
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Description

CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications include: immunocytochemistry3.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  3. Ernst CW, et al. 2007. Clin. Exp. Immunol. 148:271. (ICC) PubMed
Product Citations
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  2. Martínez-Sabadell A, et al. 2022. Cell Rep. 41:111430. PubMed
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  4. Ezeonwumelu IJ, et al. 2022. Int J Mol Sci. 23: . PubMed
  5. Wang P, et al. 2023. Front Immunol. 13:1104329. PubMed
  6. Zhang B, et al. 2023. Signal Transduct Target Ther. 8:28. PubMed
  7. Cao B, et al. 2022. Nat Commun. 13:6203. PubMed
  8. Siepe DH, et al. 2022. Elife. 11:. PubMed
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  10. Wu X, et al. 2023. iScience. 26:106559. PubMed
  11. Beckinger S, et al. 2023. Front Oncol. 13:1160824. PubMed
  12. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  13. Fuhrman C, et al. 2015. J Immunol. 195: 145 - 155. PubMed
  14. Goncharov MM, et al. 2022. Elife. 11:. PubMed
  15. Bednarski J, et al. 2016. J Exp Med. 213: 209 - 223. PubMed
  16. Pedersen JG, et al. 2021. Front Microbiol. 12:763030. PubMed
  17. Nixon CC, et al. 2020. Cell Reports Medicine. 578(7793):160-165. PubMed
  18. Dean JW, et al. 2020. J Autoimmun. 108:102417. PubMed
  19. Alenazy MF, et al. 2021. Sci Rep. 11:5629. PubMed
  20. Wang J, et al. 2020. Cell. 183:1264. PubMed
  21. Soodgupta D, et al. 2019. Cell Rep. 29:829. PubMed
  22. Juno JA, et al. 2020. Nat Med. 26:1428. PubMed
  23. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  24. Shevyrev D, et al. 2021. Exp Ther Med. 209:21. PubMed
  25. Cheng B, et al. 2022. Cancer Commun (Lond). 42:17. PubMed
  26. Walter F, et al. 2020. PLoS One. 15:e0239369. PubMed
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  28. Luo Y, et al. 2021. Nat Commun. 12:3913. PubMed
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  33. Ye Z, et al. 2021. NPJ Aging Mech Dis. 7:4. PubMed
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  35. Wallstabe J, et al. 2020. ALTEX. 37:429. PubMed
  36. Pahl JHW, et al. 2018. Cancer Immunol Res. 0.609027778. PubMed
  37. Burke MT, et al. 2017. Transplant Direct. 3:e171. PubMed
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  39. Winkler C, et al. 2021. JCI Insight. 6:. PubMed
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  41. Hu JQ, et al. 2020. J Cancer. 3.409722222. PubMed
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  44. Yamaguchi K, et al. 2018. Cancer Sci. 109:3032. PubMed
RRID
AB_314279 (BioLegend Cat. No. 302609)
AB_314279 (BioLegend Cat. No. 302610)

Antigen Details

Structure
Type I transmembrane glycoprotein, 55 kD
Distribution

Activated T cells and B cells, monocytes/macrophages, Treg

Function
Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex
Ligand/Receptor
IL-2
Cell Type
B cells, Macrophages, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann T. 1991. J. Biol. Chem. 266:2681.

Gene ID
3559 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org
Go To Top Version: 3    Revision Date: 12/15/2021

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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