APC anti-human CD314 (NKG2D) Antibody

Pricing & Availability
Clone
1D11 (See other available formats)
Regulatory Status
RUO
Other Names
NKG2D
Isotype
Mouse IgG1, κ
1D11_APC_022006
Human peripheral blood lymphocytes were stained with CD314 (clone 1D11) APC and CD56 PE.
  • 1D11_APC_022006
    Human peripheral blood lymphocytes were stained with CD314 (clone 1D11) APC and CD56 PE.
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320807 25 tests $139.00
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320808 100 tests $273.00
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Description

CD314 is a homodimeric C-type lectin-like protein also known as NKG2D. It is expressed on NK cells, CD8+ T cells, γ/δ T cells, and in vitro induced LAK cells. Several molecules have been identified as the ligands for NKG2D, including MHC class-I chain-related protein A (MICA), MICB, and UL16-binding proteins (ULBPs). NKG2D has no intrinsic signaling capacity, but attains this by non-covalent association with DAP10 or DAP12 adaptors. In addition to being a primary activation receptor on NK cells, NKG2D is also a costimulatory receptor for TCR-mediated T cell proliferation and cytokine production. The interaction of NKG2D with its ligands plays a role in the immune surveillance against pathogen and tumor cells, and in the pathogenesis of autoimmune diseases.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

The 1D11 antibody blocks MICA binding to T cells, induces redirected lysis, and costimulates T cells activation and proliferation. Additional reported (for the relevant formats) applications include: immunoprecipitation1,2, blocking of ligand binding, induction of redirected cell lysis, and costimulation of T cells proliferation2-7. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 320814) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered.

Application References

(PubMed link indicates BioLegend citation)
  1. Wu J, et al. 1999. Science 285:730.
  2. Wu J, et al. 2000. J. Exp. Med. 192:1059.
  3. Groh V, et al. 2001. Nature Immunol. 2:255.
  4. Wu J, et al. 2002. J. Immunol. 169:1236.
  5. Roberts A, et al. 2001. J. Immunol. 167:5527.
  6. Groh V, et al. 2003. Proc. Natl. Acad. Sci. USA 100:9452.
  7. Kraetzel K et al. 2008. Eur. Respir. J. 32:563. PubMed
  8. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
  9. Watanbe M, et al. 2014. Int Immunol. PubMed
Product Citations
  1. Stitzlein LM, et al. 2023. Front Neurol. 14:1112207. PubMed
  2. Wang H, et al. 2022. Front Immunol. 13:852436. PubMed
  3. Jeon SB, et al. 2022. Int J Stem Cells. Online ahead of print. PubMed
  4. Wang M, et al. 2022. Immun Inflamm Dis. 10:e626. PubMed
  5. Chen M, et al. 2021. Cancers (Basel). 13:. PubMed
  6. Gao Y, et al. 2021. Oncogenesis. 10:62. PubMed
  7. Watanabe M, et al. 2014. Int Immunol. 26:597. PubMed
  8. de Jonge K et al. 2019. Scientific reports. 9(1):4487 . PubMed
  9. Welsh JA, et al. 2022. Cell Rep Methods. 2:100136. PubMed
  10. Wu X, et al. 2020. Cancers (Basel). 12:. PubMed
  11. Sun B, et al. 2019. Cancer Immunol Res. 1.550694444. PubMed
  12. Paidipally P, et al. 2009. J Immunol. 183:1940. PubMed
  13. Bigler M, et al. 2015. PLoS One. 10: 0145635. PubMed
  14. Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed
  15. Wu X, et al. 2021. Front Immunol. 12:731767. PubMed
  16. Bermudez-Brito M, Faas M, Vos P 2016. Hum Reprod. 31: 1462 - 1474. PubMed
  17. Bonte S, et al. 2021. Oncoimmunology. 10:1954800. PubMed
  18. Oyer JL, et al. 2022. Front Immunol. 13:861681. PubMed
  19. Pahl JHW, et al. 2018. Cancer Immunol Res. 0.609027778. PubMed
  20. Wu J, et al. 2021. Adv Sci (Weinh). 8:e2101029. PubMed
  21. Lu H, et al. 2020. Am J Reprod Immunol. 83:. PubMed
  22. Wang S, et al. 2022. Cancer Immunol Immunother. Online ahead of print. PubMed
RRID
AB_492962 (BioLegend Cat. No. 320807)
AB_492962 (BioLegend Cat. No. 320808)

Antigen Details

Structure
C-type lectin
Distribution

NK cells, γ/δ T cells, CD8+ T cells

Function
Cytolytic killing of target cells expressing NKG2D ligands, costimulation of NK cells and T cells
Ligand/Receptor
MICA, MICB, UL16-binding proteins (ULBPs)
Cell Type
NK cells, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Vance RE, et al. 1999. J. Exp. Med. 190:1801.
2. Raulet DH. 2003. Nat. Rev. Immunol. 3:781.
3. Lohwasser S, et al. 1999. Eur. J. Immunol. 29:755.
4. Jamieson AM, et al. 2002. Immunity 17:19.
5. Gilfillan S, et al. 2002. Nat. Immunol. 3:1150.
6. Ho EL, et al. 2002. J. Immunol. 169:3667.
7. Maasho K, et al. 2005. J. Immunol. 174:4480.
8. Groh V, et al. 2003. Proc. Natl. Acad. Sci. USA 100:9452.

Gene ID
22914 View all products for this Gene ID
UniProt
View information about CD314 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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