APC anti-human CD45 Antibody

Pricing & Availability
Clone
2D1 (See other available formats)
Regulatory Status
RUO
Other Names
Leukocyte Common Antigen (LCA), T200
Isotype
Mouse IgG1, κ
2D1_APC_CD45_Antibody_FC_102215
Human peripheral blood lymphocytes were stained with CD45 (clone 2D1) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
  • 2D1_APC_CD45_Antibody_FC_102215
    Human peripheral blood lymphocytes were stained with CD45 (clone 2D1) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
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368511 25 tests $78.00
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368512 100 tests $220.00
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Description

CD45 is a 180 - 240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes or platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human PBMC
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.

Application References

(PubMed link indicates BioLegend citation)
  1. Bradstock KF, et al. 1980. J. Natl. Cancer Inst. 65:33.
  2. Csiba A, et al. 1984. Br. J. Cancer 50:699.
  3. Tchilian EZ, et al. 2001. J. Immunol. 166:1308.
  4. Lee MS, et al. 2004. Int. Immunol. 16:1109.
Product Citations
  1. Zhou Y, et al. 2020. Cancer Cell. 38(6):818-828.e5. PubMed
  2. Govaerts I, et al. 2021. J Hematol Oncol. 14:97. PubMed
  3. Voit RA, et al. 2023. Nat Immunol. 24:69. PubMed
  4. Issa N, et al. 2023. Leukemia. :. PubMed
  5. Lee CW, et al. 2022. J Adv Res. 39:73. PubMed
  6. Park J, et al. 2022. Bone Joint Res. 11:777. PubMed
  7. Campbell KA, et al. 2023. Commun Biol. 6:264. PubMed
  8. Galán-Díez M, et al. 2022. Cancer Discov. . PubMed
  9. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  10. Azizi E et al. 2018. Cell. 174(5):1293-1308 e36. PubMed
  11. Marinaccio C, et al. 2021. Cancer Discovery. 11(6):1398-1410. PubMed
  12. Jiang S, et al. 2021. Bioact Mater. 2711:6. PubMed
  13. Kim JT, et al. 2022. Nat Commun. 13:121. PubMed
  14. Antonucci L, et al. 2020. Journal of Immunology Research. 3257.929861. PubMed
  15. Hansen IS, et al. 2018. Nat Commun. 9:863. PubMed
  16. Liu S, et al. 2021. Mol Ther. 29:1918. PubMed
  17. Choi J, et al. 2021. Nat Cell Biol. 23:953. PubMed
  18. Celik H, et al. 2021. Cancer Discov. 11:3126. PubMed
  19. Gao P, et al. 2017. Cytotechnology. 69:751. PubMed
  20. Chen D, et al. 2021. Molecular Cell. 81:3833. PubMed
  21. Qian SJ, et al. 2021. Front Immunol. 12:711337. PubMed
  22. Li J, et al. 2021. Mol Med Rep. 1:23. PubMed
  23. Wegner J, et al. 2021. Sci Rep. 11:14983. PubMed
  24. Sun J et al. 2018. Cell stem cell. 23(3):355-369 . PubMed
  25. Yoshida R, et al. 2022. Cancer Res. :. PubMed
  26. Lee JK, et al. 2021. Cells. 10:. PubMed
  27. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  28. Lin KH, et al. 2020. Nat Genet. 52:408. PubMed
  29. Krivtsov AV, et al. 2020. Cancer Cell. 36(6):660-673.e11.. PubMed
  30. Dou D, et al. 2016. Nat Cell Biol. 18: 595-606. PubMed
  31. Wang J, et al. 2020. Cell Stem Cell. 27(1):81-97. PubMed
  32. Yang X, et al. 2020. Immunity. 51(6):983-996.e6.. PubMed
  33. Mahajan A, et al. 2019. J Leukoc Biol. 105:1087. PubMed
  34. Chijimatsu R, et al. 2022. Regen Ther. 21:52. PubMed
RRID
AB_2566371 (BioLegend Cat. No. 368511)
AB_2566371 (BioLegend Cat. No. 368512)

Antigen Details

Structure
Type I transmembrane protein, 180 - 240 kD (multiple isoforms)
Distribution

Hematopoietic cells, except circulating erythrocytes or platelets.

Function
Tyrosine phosphatases, signaling, co-stimulation (co-inhibition), TCR and BCR mediated activation.
Ligand/Receptor
Galectin-1, CD2, CD3, and CD4.
Cell Type
B cells, Dendritic cells, Neutrophils
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Protein Kinases/Phosphatase, TCRs
Antigen References

1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol. 12:85.

Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org
Go To Top Version: 1    Revision Date: 10/23/2015

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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