APC anti-human CD86 Antibody

Pricing & Availability
Clone
IT2.2 (See other available formats)
Regulatory Status
RUO
Workshop
VI CD86.8
Other Names
B7-2, B70
Isotype
Mouse IgG2b, κ
IT2dot2_APC_111607
Human peripheral blood monocytes stained with IT2.2 APC
  • IT2dot2_APC_111607
    Human peripheral blood monocytes stained with IT2.2 APC
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305411 25 tests $149.00
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305412 100 tests $314.00
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Description

CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, monocytes/macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is the ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce costimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can bind to CD152 as well, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Technical data sheet

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Capuchin Monkey, Common Marmoset, Cotton-topped Tamarin, Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6, Western blotting3, and blocking of T cell activation2,4,5. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 305449 & 305450).

Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  2. Dieu M. 1998. J. Exp. Med. 188:373. (Block)
  3. Esser M, et al. 2001. J. Virol. 75:6173. (WB)
  4. Jeannin P, et al. 1999. J. Immunol. 162:2044. (Block)
  5. Kapsogeorgou EK, et al. 2001. J. Immunol. 166:3107. (Block)
  6. Geissmann F, et al. 2001. Blood 97:1241. (IHC)
Product Citations
  1. Tu J, et al. 2022. Cancer Sci. 113:2496. PubMed
  2. Taffoni C 2022. The EMBO Journal. e111961. PubMed
  3. Ackermann M, et al. 2018. Nat Commun. 9:5088. PubMed
  4. Rodriguez-Perdigon M, et al. 2023. Front Bioeng Biotechnol. 11:1159819. PubMed
  5. Chometon TQ, et al. 2020. PLoS One. 15:e0231132. PubMed
  6. Vereertbrugghen A, et al. 2021. Front Oncol. 11:598319. PubMed
  7. Meng Y,et al. 2017. Cell Death Dis. . 10.1038/cddis.2017.505. PubMed
  8. Herath S, et al. 2014. PLoS One. 9:88327. PubMed
  9. Kotsiou E, et al. 2016. Blood. 128: 72 - 81. PubMed
  10. Ong SM, et al. 2019. Front Immunol. 10:1761. PubMed
  11. Xu G, et al. 2022. Cell Death Dis. 13:888. PubMed
  12. Hu X, et al. 2016. PLoS One. 11: 0152368. PubMed
  13. Castellaro AM, et al. 2019. Cancers (Basel). 11:. PubMed
  14. Cassetta L et al. 2019. Cancer Cell. 35(4):588-602 . PubMed
  15. Kim SE, et al. 2019. PLoS One. 14:e0223590. PubMed
  16. Arcangeli S, et al. 2022. J Clin Invest. 132: . PubMed
  17. Muto S, et al. 2021. Oncol Lett. 21:203. PubMed
  18. Hazeldine J, et al. 2017. PLoS Med. 10.1371/journal.pmed.1002338. PubMed
  19. Amo L, et al. 2022. Front Immunol. 13:835527. PubMed
  20. Perera MR, et al. 2022. Int J Mol Sci. 23:. PubMed
  21. Nowak A, et al. 2015. Ann Oncol. 26: 2483 - 2490. PubMed
  22. Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed
  23. Celis‐Gutierrez J et al. 2019. Cell Rep. 27(11):3315-3330 . PubMed
  24. Longbrake EE, et al. 2018. Mult Scler. 24:728. PubMed
  25. Zhu F, et al. 2022. Front Immunol. 13:879575. PubMed
  26. Nastasi C, et al. 2015. Sci Rep. . PubMed
  27. Papalexi E, et al. 2021. Nat Genet. 53:322. PubMed
  28. Siedlik J, et al. 2017. J Immunol Methods. 10.4049/jimmunol.1700003. PubMed
  29. Pinto SM, et al. 2021. Front Immunol. 12:679458. PubMed
RRID
AB_493231 (BioLegend Cat. No. 305411)
AB_493231 (BioLegend Cat. No. 305412)

Antigen Details

Structure
Ig superfamily, single-chain transmembrane glycoprotein, 80 kD
Distribution

Monocytes/macrophages, activated B cells and T cells, dendritic cells

Function
T cell costimulation
Ligand/Receptor
CD28, CD152
Cell Type
B cells, Dendritic cells, Macrophages, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Hathcock K, et al. 1996. Adv. Immunol. 62:131.
2. June C, et al. 1994. Immunol. Today 15:321.

Gene ID
942 View all products for this Gene ID
UniProt
View information about CD86 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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