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Human peripheral blood leukocytes stained with APC anti-Human Lineage Cocktail (CD3, CD14, CD19, CD20, CD56) and HLA-DR PerCP. -
Human Lineage negative/dim and HLA-DR+ leukocyte population was stained with CD11c FITC and CD16 PE.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 348703 | 50 tests | $622.00 | |||
This anti-Human Lineage Cocktail is optimized for the detection of human lymphocytes, monocytes, eosinophils, and neutrophils. In combination with other markers, it can be used for studies of dendritic cells (DCs), including DCs that express CD16. This cocktail is composed of CD3, CD14, CD19, CD20, and CD56. CD3 is the antigen mainly found on T cells; CD14 is expressed on monocytes/macrophages and at low levels on neutrophils and eosinophils; CD19 and CD20 are on B cells; CD56 is located on NK cells.
Product Details
- Verified Reactivity
- Human
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- Preparation
- The antibody cocktail was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
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Red Laser (633 nm)
- Product Citations
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- RRID
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AB_3076251 (BioLegend Cat. No. 348703)
Antigen Details
- Distribution
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CD3 is expressed on all T lymphocytes. CD14 is expressed on monocytes, macrophages, neutrophils, and eosinophils. CD19 and CD20 are expressed on B lymphocytes. CD56 is expressed on activated and resting NK lymphocytes.
- Cell Type
- Dendritic cells, Eosinophils, Macrophages, Mesenchymal Stem Cells, Monocytes, Neutrophils, T cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
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1. Zola H, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules. New Jersey.
2. Olweus J, et al. 1997. P. Natl. Acad. Sci. USA 94:12551.
3. Banchereau J, et al. Eds. 1995. Advances in Experimental Medicine and Biology. New York.
4. Markowicz S, et al. 1990. J. Clin. Invest. 85:955.
5. Freudenthal P, et al. 1990. P. Natl. Acad. Sci. USA 87:7698.
6. Macdonald K, et al. 2002. Blood 100:4512.
7. Piccioli D, et al. 2007. Blood 109:5371. - Gene ID
- 915 View all products for this Gene ID 930 View all products for this Gene ID 931 View all products for this Gene ID 4684 View all products for this Gene ID 929 View all products for this Gene ID
- UniProt
- View information about Lineage on UniProt.org
Other Formats
View All Lineage Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| FITC anti-human Lineage Cocktail (CD3, CD14, CD19, CD20, CD56) | UCHT1; HCD14; HIB19; 2H7; HCD56 | FC |
| APC anti-human Lineage Cocktail (CD3, CD14, CD19, CD20, CD56) | UCHT1; HCD14; HIB19; 2H7; HCD56 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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FITC anti-human Lineage Cocktail (CD3, CD14, CD19, CD20, CD56)
Human peripheral blood leukocytes stained with FITC anti-Hum... 
Human peripheral blood co-stained with CD11c APC and CD16 PE... -
APC anti-human Lineage Cocktail (CD3, CD14, CD19, CD20, CD56)
Human peripheral blood leukocytes stained with APC anti-Huma... 
Human Lineage negative/dim and HLA-DR+ leukocyte ...