APC anti-mouse CD314 (NKG2D) Antibody

Pricing & Availability
Clone
CX5 (See other available formats)
Regulatory Status
RUO
Other Names
CD314
Isotype
Rat IgG1, κ
CX5_APC_110308.jpg
C57BL/6 splenocytes stained with DX5 PE and CX 5 APC
  • CX5_APC_110308.jpg
    C57BL/6 splenocytes stained with DX5 PE and CX 5 APC
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130211 25 µg $90.00
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130212 100 µg $308.00
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Description

This antibody has been reported to block binding of NKG2D to its ligands, RAE-1 and H60, and inhibition of NKG2D-dependent NK-cell cytotoxicity.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse NKG2D
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

To reduce non-specific binding, preincubate cells with purified anti-mouse CD16/CD32, clone 93 (Cat No 101301/2) is recommended.

 

This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes contact The Regents of the University of California.

Application References

(PubMed link indicates BioLegend citation)
  1. Ogasawara K, et al. 2003. Immunity 18:41. (Block)
  2. Ogasawara K, et al. 2004. Immunity 20:757. (Block)
  3. Kong LY, et al. 2010. Clin. Cancer Res. 16:2550. PubMed
Product Citations
  1. Glasner A,et al. 2017. Sci Rep.. 10.1038/s41598-017-12998-w. PubMed
  2. Li Y, et al. 2014. PLoS One. 9:86927. PubMed
  3. Trotta E, et al. 2018. Nat Med. 24:1005. PubMed
  4. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  5. Mah-Som AY, et al. 2021. Cell Reports. 35(9):109209. PubMed
  6. Theurich S et al. 2017. Cell metabolism. 26(1):171-184 . PubMed
  7. Seo IH, et al. 2021. Cell Reports. 36(4):109438. PubMed
  8. Zhou X, et al. 2021. Nutr Metab (Lond). 18:64. PubMed
RRID
AB_1236372 (BioLegend Cat. No. 130211)
AB_1236372 (BioLegend Cat. No. 130212)

Antigen Details

Structure
NKG2D is a lectin-like type II transmembrane protein. It serves as a stimulatory receptor to activate NK cells via the non-covalently associated DAP10 or DAP 12 daptor protein.
Distribution

NK, NKT cells

Ligand/Receptor
RAE, H60,
Cell Type
NK cells, NKT cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Gene ID
27007 View all products for this Gene ID
UniProt
View information about CD314 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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