- Clone
- 4G2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- 19A, CS1, CD319, CRACC, SLAMF7
- Isotype
- Rat IgG1, κ
-
C57BL/6 mouse splenocytes were stained with CD19 FITC and CD319 APC (top) or rat IgG1, κ isotype control APC (bottom). -
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 152003 | 25 µg | $166.00 | |||
| 152004 | 100 µg | $374.00 | |||
CD319/CD319/CS1/CRACC/SLAMF7 is a 66 kD transmembrane protein belonging to the CD2 subset of the immunoglobulin superfamily; it is expressed on most NK cells, B cells, and subsets of T cells. CD319 activates immune responses in NK cells when coupled with SAP-family adaptors (SAP, EAT-2, ERT) and inhibits immune responses in the absence of SAP-family adaptors or when bound to SH-domain molecules.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- CD319-Fc fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Red Laser (633 nm)
- RRID
-
AB_2632675 (BioLegend Cat. No. 152003)
AB_2632675 (BioLegend Cat. No. 152004)
Antigen Details
- Structure
- Extracellular domain with one Ig like V-set domain and one Ig like C2-set domain, and an cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs ITSMs.
- Distribution
-
Spleen, lymph node, bone marrow, testis, thymus; NK cells, B cells, and subsets of T cells.
- Function
- Activates immune responses in NK cells when coupled with SAP-family adaptors (SAP, EAT-2, ERT).
- Interaction
- Interacts (via ITSM phosphorylated on Tyr-302) with SH2D1B, PTPN6/SHP-1, PTPN11/SHP-2, INPP5D/SHIP1, CSK and FYN, and SAP-family adaptors (SAP, EAT-2, ERT).
- Cell Type
- B cells, NK cells, T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Li Y, et al. 2013. PLoS One 8(9):e76681.
2. Dongre P, et al. 2013. Mol. Immunol. 54:254-63.
3. Tovar V, et al. 2002. Immunogenetics 54:394.
4. Dong Z, et al. 2009. Nat. Immunol. 10:973. - Gene ID
- 75345 View all products for this Gene ID
- UniProt
- View information about CD319 on UniProt.org
Other Formats
View All CD319 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse CD319 | 4G2 | FC,Direct ELISA,WB |
| PE anti-mouse CD319 | 4G2 | FC |
| APC anti-mouse CD319 | 4G2 | FC |
| TotalSeq™-C1265 anti-mouse CD319 | 4G2 | PG |
| TotalSeq™-B1265 anti-mouse CD319 | 4G2 | PG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD319
C57BL/6 mouse splenocytes were stained with purified CD319 (... 
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PE anti-mouse CD319
C57BL/6 mouse splenocytes were stained with CD19 FITC and CD... 
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APC anti-mouse CD319
C57BL/6 mouse splenocytes were stained with CD19 FITC and CD... 
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TotalSeq™-C1265 anti-mouse CD319
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TotalSeq™-B1265 anti-mouse CD319