APC anti-mouse CD357 (GITR) Antibody

Pricing & Availability
Clone
DTA-1 (See other available formats)
Regulatory Status
RUO
Other Names
Glucocorticoid-induced TNFR -related gene, TNFRSF18
Isotype
Rat IgG2b, κ
DTA-1_APC_100809
C57BL/6 splenocytes stained with DTA-1 APC
  • DTA-1_APC_100809
    C57BL/6 splenocytes stained with DTA-1 APC
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Cat # Size Price Quantity Check Availability
126311 25 µg $138.00
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126312 100 µg $325.00
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Description

GITR (glucocorticoid-induced TNFR-related gene) is a member of the TNF receptor superfamily, also known as TNFRSF18 and AITR (in humans). It is expressed at low levels on resting T lymphocytes and at high levels on CD25+ CD4+ Tregs. The expression of GITR on T cells can be upregulated upon activation. Interaction of GITR with its ligand (GITRL) has been demonstrated to augment T cell activation, proliferation, cytokine production as well as MAPKs and NF-κB activation, and abrogate the inhibitory function of CD25+ CD4+ Tregs. In vivo activation of GITR causes development of autoimmune diseases and restores the suppressed immune response.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD25+ CD4+ T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. De Luca A, et al. 2007. J. Immunol. 179:5999. PubMed
Product Citations
  1. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  2. Saxena V, et al. 2022. Cell Rep. 39:110727. PubMed
  3. Yazicioglu YF, et al. 2023. Nat Immunol. 24:991. PubMed
  4. Alissafi T, et al. 2018. J Clin Invest. 128:3840. PubMed
  5. Alissafi T, et al. 2020. Cell Metabolism. 32(4):591-604.e7. PubMed
  6. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
  7. Cheng B, et al. 2022. Cancer Commun (Lond). 42:17. PubMed
  8. Knizkova D, et al. 2022. Nat Immunol. 23:1644. PubMed
  9. Katagiri T, et al. 2019. Mucosal Immunol. 12:p1104. PubMed
  10. Fan MY et al. 2018. Cell reports. 25(5):1204-1213 . PubMed
RRID
AB_1134212 (BioLegend Cat. No. 126311)
AB_1134212 (BioLegend Cat. No. 126312)

Antigen Details

Structure
66-70 kD homodimer of TNFR superfamily member.
Distribution

Low levels on resting T cells, B cells, and macrophages; high levels on CD25+ CD4+ Tregs and CD25+ CD4+ CD8- thymocytes.

Function
Stimulation of GITR can abrogate or reduce the inhibitory function of CD25+ CD4+ Treg cells. In vivo activation of GITR may lead to the development of autoimmune diseases.
Ligand/Receptor
GITR ligand (GITRL).
Cell Type
B cells, Macrophages, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Tone M, et al. 2003. Proc. Natl. Acad. Sci. USA 100:15059.
2. Shimizu J, et al. 2002 Nat. Immunol. 3:135.
3. Stephens GL, et al. 2004. J. Immunol. 173:5008.
4. McHugh RS, et al. 2002. Immunity 16:311.

Regulation
Activation of T and B cells can upregulate GITR expression.
Gene ID
21936 View all products for this Gene ID
UniProt
View information about CD357 on UniProt.org
Go To Top Version: 2    Revision Date: 10/29/2021

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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