APC anti-mouse CD3ε Antibody

Pricing & Availability
Clone
KT3.1.1 (See other available formats)
Regulatory Status
RUO
Other Names
CD3e, T3e
Isotype
Rat IgG2a, κ
KT311_APC_CD3epsilon_Antibody_090718
C57BL/6 mouse splenocytes were stained with FITC anti-mouse CD19 and with PE anti-mouse CD3ε (clone KT3.1.1; left) or rat IgG2a, κ isotype control (right).
  • KT311_APC_CD3epsilon_Antibody_090718
    C57BL/6 mouse splenocytes were stained with FITC anti-mouse CD19 and with PE anti-mouse CD3ε (clone KT3.1.1; left) or rat IgG2a, κ isotype control (right).
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Cat # Size Price Quantity Check Availability
155605 25 µg $36.00
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155606 100 µg $113.00
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Description

CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CBAT6 Thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Tomonari K. 1988. Immunogenetics. 28:455. (FC)
Product Citations
  1. Zhao Q, et al. 2023. Nutrients. 15: . PubMed
RRID
AB_2750431 (BioLegend Cat. No. 155605)
AB_2750431 (BioLegend Cat. No. 155606)

Antigen Details

Structure
Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ζ subunits and TCR (α/β and γ/δ), 20 kD.
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
TCR signal transduction, T cell activation
Ligand/Receptor
TCR
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References
  1. Preston S, et al. 1997. Eur. J. Immunol. 27:1911.
  2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
  3. Weiss A, Littman DR. 1994. Cell. 76:263.
Gene ID
12501 View all products for this Gene ID
UniProt
View information about CD3epsilon on UniProt.org
Go To Top Version: 1    Revision Date: 09/10/2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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