APC anti-mouse CD94 Antibody

Pricing & Availability
Clone
18d3 (See other available formats)
Regulatory Status
RUO
Other Names
Kp43
Isotype
Rat IgG2a, κ
18d3_APC_CD94_Antibody_1_122617
C57BL/6 mouse splenocytes were stained with CD49b (pan-NK cells) FITC and CD94 (clone 18d3) APC (top) or rat IgG2a, κ APC isotype control (bottom).
  • 18d3_APC_CD94_Antibody_1_122617
    C57BL/6 mouse splenocytes were stained with CD49b (pan-NK cells) FITC and CD94 (clone 18d3) APC (top) or rat IgG2a, κ APC isotype control (bottom).
  • 18d3_APC_CD94_Antibody_2_122617
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability
105511 25 µg $149.00
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105512 100 µg $350.00
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Description

CD94 is a 43/39 kD C-type lectin, also known as Kp43. It is present on all NK cells, NKT cells, and a subset of CD8-positive T lymphocytes in most mouse strains. CD94 is a type-II transmembrane protein with an extracellular lectin-like domain and a short cytoplasmic tail. CD94 is expressed as a disulphide-linked heterodimer with a NKG2 subunit believed to mediate signal transduction. When associated with NKG2A, the complex triggers inhibition; when associated with NKG2C, the complex triggers stimulation. The receptor complex of CD94 and NKG2 receptors bind to the ligand, Qa-1, and are thought to play a role in maintaining self-tolerance in developing NK cells.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CHO cells expressing the C57BL/6 alleles of CD94 and NKG2A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Vance RE, et al. 1999. J. Exp. Med. 190:1801.
  2. Tang X, et al. 2006. J. Immunol. 177:7645. PubMed
RRID
AB_2721459 (BioLegend Cat. No. 105511)
AB_2721459 (BioLegend Cat. No. 105512)
Disclaimer

This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.

Antigen Details

Structure
C-type lectin; 43/39 kD
Distribution

NK cells, NK-T cells, CD8+ T subset

Function
Inhibits NK cell function
Ligand/Receptor
CD94/NKG2
Cell Type
NK cells, NKT cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References
  1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
  2. Lopez-Botet M, et al. 1997. Immunol. Rev. 155:165.
  3. Moretta A, et al. 1997. Immunol. Rev. 155:105.
  4. Phillips JH, et al. 1996. Immunity 5:163.
Gene ID
16643 View all products for this Gene ID
UniProt
View information about CD94 on UniProt.org
Go To Top Version: 1    Revision Date: 12/26/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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