APC anti-mouse/rat/human MCP-1 Antibody

Pricing & Availability
Clone
2H5 (See other available formats)
Regulatory Status
RUO
Other Names
Monocyte chemoattractant protein-1, Monocyte chemoattractant and activating factor (MCAF), JE, Small inducible cytokine A2 (SCYA2), HC-11, P6, Smooth muscle cell chemotactic factor (SMC-CF), CCL2
Isotype
Hamster IgG
2H5_APC_MCP-1_Antibody_ICFC_1_092415
LPS-stimulated human peripheral blood monocytes were surface stained with CD14 FITC and then intracellular stained with MCP-1 (clone 2H5) APC (top) or Armenian hamster IgG APC Isotype control (bottom).
  • 2H5_APC_MCP-1_Antibody_ICFC_1_092415
    LPS-stimulated human peripheral blood monocytes were surface stained with CD14 FITC and then intracellular stained with MCP-1 (clone 2H5) APC (top) or Armenian hamster IgG APC Isotype control (bottom).
  • 2H5_APC_MCP-1_Antibody_ICFC_2_092415
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability
505909 25 µg $102.00
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505910 100 µg $243.00
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Description

Monocyte chemotactic protein-1 (MCP-1) also known as monocyte chemotactic and activating factor (MCAF) was identified based on its ability to chemoattract monocytes. Subsequently, MCP-1 has also been found to regulate adhesion molecule expression and cytokine production in monocytes. MCP-1 is identical to the product of the JE gene, a PDGF inducible gene. MCP-1 is a member of the beta (C-C) chemokine subfamily, known as CCL2. The 2H5 antibody reacts with mouse, rat, and human MCP-1. The 2H5 antibody can neutralize the bioactivity of natural or recombinant MCP-1.

Technical data sheet

Product Details

Verified Reactivity
Mouse, Rat, Human
Reported Reactivity
Rhesus
Antibody Type
Monoclonal
Host Species
Hamster
Immunogen
CHO-expressed, recombinant mouse MCP-1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

ELISA or ELISPOT Capture1: The Ultra-LEAF™ 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 4E2/MCP antibody (Cat. No. 506002) as the detecting antibody for the detection of mouse MCP-1. The purified 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 5D3-F7 antibody (Cat. No. 502609) as the detecting antibody for the detection of human MCP-1.
Flow Cytometry2: The fluorochrome-labeled 2H5 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify MCP-1 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.
Neutralization1,4,6: The Ultra-LEAF™ purified antibody (Cat. Nos. 505911 & 505912) is recommended for neutralization.
Additional reported applications (for the relevant formats) include: Western blotting3, immunohistochemistry of paraformaldehyde-fixed, saponin-treated frozen tissue sections5, and acetone-fixed frozen tissue sections7.

Application References

(PubMed link indicates BioLegend citation)
  1. Luo, Y., et al. 1994. J. Immunol. 153:3708.
  2. Zhang, Y., et al. 2002. J. Immunol. 168:3088.
  3. Luo, Y., et al. 1999 J. Immunol. 163:3985.
  4. Morrison, B. E., et al. 2003 J. Clin. Invest. 112:1862.
  5. Hancock, W. W., et al. 1997 Transplantation 64:696.
  6. Yu, R., et al. 2006 Obesity 14:1353.
  7. Taal MW, et al. 2001. Am. J. Physiol. Renal. Physiol. 280:F343.
  8. Wan W, et al. 2015. Cardiovasc Res. 106:478. PubMed
Product Citations
  1. Campisi L, et al. 2022. Nature. 606:945. PubMed
  2. Jiang P, et al. 2021. Nat Methods. 18:1181. PubMed
  3. Pascual–García M, et al. 2019. Nat Commun. 10:2416. PubMed
RRID
AB_2566054 (BioLegend Cat. No. 505909)
AB_2566054 (BioLegend Cat. No. 505910)

Antigen Details

Structure
CC chemokine; dimer; 13.8 kD (Mammalian)
Bioactivity
Chemoattractant; regulates adhesion molecule expression, cytokine production in monocytes; proliferation/activation of CC-chemokine activated killer cells
Cell Sources
Monocytes, vascular endothelial cells, smooth muscle, glomerular mesangial cells
Cell Targets
Monocytes, basophils, T cells, NK cells, immature dendritic cells
Receptors
CCR2; CCR5; CCR10; D6; possibly L-CCR
Biology Area
Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Bischoff, S., et al. 1992. J. Exp. Med. 175:1271.
3. Charo, I., et al. 1994. P. Natl. Acad. Sci. USA 91:2752.
4. Jiang, Y., et al. 1992. J. Immunol. 148:2423.

Regulation
Upregulated by IL-1, TNF-α, PDGF, TGF-β
Gene ID
20296 View all products for this Gene ID 24770 View all products for this Gene ID 6347 View all products for this Gene ID
UniProt
View information about CCL2 on UniProt.org

Other Formats

View All CCL2 Reagents Request Custom Conjugation
Description Clone Applications
PE anti-mouse/rat/human MCP-1 2H5 ICFC
Purified anti-mouse/rat/human MCP-1 2H5 ELISA Capture,IHC,WB
Biotin anti-mouse/rat/human MCP-1 2H5 ELISA Detection,ELISPOT Detection
APC anti-mouse/rat/human MCP-1 2H5 ICFC
Ultra-LEAF™ Purified anti-mouse/rat/human MCP-1 2H5 ELISA Capture,ELISPOT Capture,IHC,Neut,WB
Go To Top Version: 3    Revision Date: 06/15/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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