APC/Cyanine7 anti-human CD14 Antibody

Pricing & Availability
Clone
M5E2 (See other available formats)
Regulatory Status
RUO
Workshop
III 329
Other Names
LPS receptor
Isotype
Mouse IgG2a, κ
M5E2_APCCyanine7_CD14_Antibody_010819.png
Human peripheral blood monocytes were stained with CD14 (clone M5E2) APC/Cyanine7 (filled histogram) or mouse IgG2a, κ APC/Cyanine7 isotype control (open histogram)
  • M5E2_APCCyanine7_CD14_Antibody_010819.png
    Human peripheral blood monocytes were stained with CD14 (clone M5E2) APC/Cyanine7 (filled histogram) or mouse IgG2a, κ APC/Cyanine7 isotype control (open histogram)
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability
301819 25 tests $143.00
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301820 100 tests $292.00
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Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens, and in the upregulation of adhesion molecules and expression of cytokines in monocytes and neutrophils.

Technical data sheet

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Capuchin Monkey, Cow, Chimpanzee, Common Marmoset, Cotton-topped Tamarin, Dog, Pigtailed Macaque, Squirrel Monkey
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length human CD14 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation4, and immunofluorescence microscopy5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301861 and 301862).

Application References

(PubMed link indicates BioLegend citation)
  1. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
  2. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (IHC-F)
  3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  4. Power CP, et al. 2004. J. Immunol. 173:5229. (Block)
  5. Williams KC, et al. 2001. J. Exp. Med. 193:905.
  6. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (FC) PubMed
  7. Santer DM, et al. 2010. J. Immunol. 485:4739. PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Zizzo G, et al. 2012. J. Immunol. 189:3508. PubMed
  10. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  11. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Schneider MM, et al. 2022. Life Sci Alliance. 5: . PubMed
  2. Paniskaki K, et al. 2022. Transplant Proc. 54:1455. PubMed
  3. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  4. Shishkova D, et al. 2022. Int J Mol Sci. 23: . PubMed
  5. Pozzetto B, et al. 2021. Nature. 600:701. PubMed
  6. Schmitz T, et al. 2021. J Reprod Immunol. 148:103424. PubMed
  7. Prete G, et al. 2016. Antimicrob Agents Chemother. 60: 1560 - 1572. PubMed
  8. Gajdasik DW, et al. 2020. Nat Commun. 2.834027778. PubMed
  9. Zhang B, et al. 2022. J Exp Med. 219:. PubMed
  10. Lysenko V, et al. 2020. Blood Adv. 4:2477. PubMed
  11. Krauss PL, et al. 2021. Front Immunol. 12:730672. PubMed
  12. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  13. Gleason M, et al. 2014. Blood. 123:3016. PubMed
  14. Kuehn C, et al. 2014. Pathol Biol (Paris). 62:354. PubMed
  15. Arcangeli S, et al. 2022. J Clin Invest. 132: . PubMed
  16. Muto S, et al. 2021. Oncol Lett. 21:203. PubMed
  17. Sureshchandra S, et al. 2021. iScience. 24:102690. PubMed
  18. Goods BA, et al. 2021. JCI Insight. 6: . PubMed
  19. Prete G, et al. 2014. Antimicrob Agents Chemother. 58:6790. PubMed
  20. Lee JH, et al. 2021. NPJ Vaccines. 6:113. PubMed
  21. Herati RS, et al. 2021. Cell Reports Medicine. 2(5):100262. PubMed
  22. Zabaleta N, et al. 2021. Cell Host Microbe. :. PubMed
  23. Rossignol ED, et al. 2021. Cell Reports. 35(8):109167. PubMed
  24. Janela B, et al. 2019. Immunity. 50:1069. PubMed
  25. Liu W, et al. 2022. Bio Protoc. 12:e4285. PubMed
  26. Meckiff BJ, et al. 2020. Cell. 183(5):1340-1353.e16. PubMed
  27. Prete G, et al. 2012. J Virol. 86:3152. PubMed
  28. Shemesh A, et al. 2022. J Exp Med. 219:. PubMed
  29. Cirelli KM et al. 2019. Cell. 177(5):1153-1171 . PubMed
  30. Messerer DAC, et al. 2020. Front Immunol. 11:571992. PubMed
  31. Kong XF, et al. 2018. Nat Immunol. 19:973. PubMed
  32. Rönnberg E, et al. 2019. Front Immunol. 1.361805556. PubMed
  33. Clayton KL, et al. 2021. Cell Host Microbe. 29(3):435-447.e9. PubMed
  34. Askenase MH, et al. 2021. Sci Immunol. 6:. PubMed
  35. Leboux RJT, et al. 2022. Front Allergy. 2:642788. PubMed
  36. Inglis H, et al. 2015. J Vis Exp. 97: 52484. PubMed
  37. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
RRID
AB_493694 (BioLegend Cat. No. 301819)
AB_493694 (BioLegend Cat. No. 301820)

Antigen Details

Structure
GPI-linked membrane glycoprotein, 53-55 kD
Distribution

Monocytes, macrophages, granulocytes (low)

Function
LPS receptor, clearance of Gram-negative pathogens
Ligand/Receptor
LPS
Cell Type
Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.

Gene ID
929 View all products for this Gene ID
UniProt
View information about CD14 on UniProt.org

Other Formats

View All CD14 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD14 M5E2 FC
FITC anti-human CD14 M5E2 FC
PE anti-human CD14 M5E2 FC
Purified anti-human CD14 M5E2 FC,CyTOF®,Block,IHC-F
PE/Cyanine7 anti-human CD14 M5E2 FC
Alexa Fluor® 488 anti-human CD14 M5E2 FC
Alexa Fluor® 647 anti-human CD14 M5E2 FC
Ultra-LEAF™ Purified anti-human CD14 M5E2 FC,CyTOF®,Block,IHC-F
Pacific Blue™ anti-human CD14 M5E2 FC
APC/Cyanine7 anti-human CD14 M5E2 FC
Alexa Fluor® 700 anti-human CD14 M5E2 FC
PerCP/Cyanine5.5 anti-human CD14 M5E2 FC
Biotin anti-human CD14 M5E2 FC
Brilliant Violet 421™ anti-human CD14 M5E2 FC
Brilliant Violet 570™ anti-human CD14 M5E2 FC
Brilliant Violet 605™ anti-human CD14 M5E2 FC
Brilliant Violet 650™ anti-human CD14 M5E2 FC
Brilliant Violet 711™ anti-human CD14 M5E2 FC
Brilliant Violet 785™ anti-human CD14 M5E2 FC
Brilliant Violet 510™ anti-human CD14 M5E2 FC
Purified anti-human CD14 (Maxpar® Ready) M5E2 FC,CyTOF®
PerCP anti-human CD14 M5E2 FC
FITC anti-human CD14 M5E2 FC
PE/Dazzle™ 594 anti-human CD14 M5E2 FC
Pacific Blue™ anti-human CD14 M5E2 FC
APC/Fire™ 750 anti-human CD14 M5E2 FC
APC anti-human CD14 M5E2 FC
TotalSeq™-A0081 anti-human CD14 M5E2 PG
TotalSeq™-B0081 anti-human CD14 M5E2 PG
TotalSeq™-C0081 anti-human CD14 M5E2 PG
PE anti-human CD14 M5E2 FC
PE/Cyanine5 anti-human CD14 M5E2 FC
TotalSeq™-D0081 anti-human CD14 M5E2 PG
APC/Fire™ 750 anti-human CD14 M5E2 FC
GMP FITC anti-human CD14 M5E2 FC
PE/Cyanine7 anti-human CD14 M5E2 FC
GMP APC anti-human CD14 M5E2 FC
GMP PE anti-human CD14 M5E2 FC
PE/Dazzle™ 594 anti-human CD14 M5E2 FC
GMP Pacific Blue™ anti-human CD14 M5E2 FC
GMP APC/Fire™ 750 anti-human CD14 M5E2 FC
PerCP/Cyanine5.5 anti-human CD14 M5E2 FC
Spark Violet™ 500 anti-human CD14 M5E2 FC
GMP PE/Dazzle™ 594 anti-human CD14 M5E2 FC
APC/Fire™ 810 anti-human CD14 M5E2 FC
PE/Fire™ 700 anti-human CD14 M5E2 FC
Go To Top Version: 6    Revision Date: 01/08/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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