APC/Fire™ 750 anti-human β2-microglobulin Antibody

Pricing & Availability
Clone
2M2 (See other available formats)
Regulatory Status
RUO
Other Names
β2M, β2-M, beta2-microglobulin b2-M, b2M
Isotype
Mouse IgG1, κ
2M2_APCFire750_beta2-microglobulin_Antibody_111416
Human peripheral blood lymphocytes were stained with anti-human β2-microglobulin (clone 2M2) APC/Fire™ 750 (filled histogram), or mouse IgG1, κ APC/Fire™ 750 isotype control (open histogram).
  • 2M2_APCFire750_beta2-microglobulin_Antibody_111416
    Human peripheral blood lymphocytes were stained with anti-human β2-microglobulin (clone 2M2) APC/Fire™ 750 (filled histogram), or mouse IgG1, κ APC/Fire™ 750 isotype control (open histogram).
Compare all formats See APC/Fire™ 750 spectral data
Cat # Size Price Quantity Check Availability
316313 25 tests $176.00
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316314 100 tests $382.00
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Description

β2-microglobulin (β2M) is a 12 kD nonpolymorphic Ig like protein. It is a non-membrane-anchored glycoprotein and is noncovalently associated with 39-44 kD polymorphic heavy chains of MHC class I molecules to form HLA class I antigen complex. In association with HLA class I, β2M is expressed on all leukocytes, platelets, endothelial cells, and epithelial cells. β2M plays an essential role both in governing MHC class I molecules stability and in promoting antigen binding and presenting the antigen to CD3/TCR complex of CD8+ T cells.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus, Pig
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified human β2-microglobulin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.

Application Notes

Additional reported applications (for the relevant formats) include: Western blotting, and ELISA.

Application References

(PubMed link indicates BioLegend citation)
  1. Meissner TB, et al. 2010. Proc Natl Acad Sci USA. PubMed
  2. Rizvi SM, et al. 2011. J. Immunol. 186:2309. PubMed
  3. Meissner TB, et al. 2012. J Immunol. 188:4951. PubMed.
Product Citations
  1. Caution K, et al. 2019. Front Immunol. 2519:10. PubMed
RRID
AB_2632826 (BioLegend Cat. No. 316313)
AB_2632826 (BioLegend Cat. No. 316314)

Antigen Details

Structure
Nonpolymorphic Ig like structure, noncovalently associated with heavy chain of MHC class I molecules, 12 kD
Distribution

Leukocytes, platelets, endothelial cells, epithelial cells

Function
Govern MHC class I molecule stability, promote MHC class I molecules binding and presenting peptide antigens to CD8+ T cells
Ligand/Receptor
CD3/TCR complex, CD8
Cell Type
Endothelial cells, Epithelial cells, Leukocytes, Platelets
Biology Area
Immunology
Molecular Family
MHC Antigens
Antigen References

1. Engelhard VH. 1994. Curr. Opin. Immunol. 6:13.
2. Williams DB, et al. 1989. J. Immunol. 142:2796.
3. Danliczyk UG and TL. Delovitch. 1994. J. Immunol. 153:3533.
4. Williams A, et al. 2002. Tissue Antigens 59:3.

Gene ID
567 View all products for this Gene ID
UniProt
View information about beta2-microglobulin on UniProt.org
Go To Top Version: 2    Revision Date: 11/15/2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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