APC/Fire™ 750 anti-mouse CD20 Antibody

Pricing & Availability
Clone
SA271G2 (See other available formats)
Regulatory Status
RUO
Other Names
Ms4a1, Ly-44
Isotype
Rat IgG2b, κ
SA271G2_APCF750_CD20_Antibody_030122
C57BL/6 mouse splenocytes were stained with anti-mouse CD45R/B220 FITC and anti-mouse CD20 (clone SA271G2) APC/Fire™ 750 (left) or rat IgG2b, κ APC/Fire™ 750 isotype control (right).
  • SA271G2_APCF750_CD20_Antibody_030122
    C57BL/6 mouse splenocytes were stained with anti-mouse CD45R/B220 FITC and anti-mouse CD20 (clone SA271G2) APC/Fire™ 750 (left) or rat IgG2b, κ APC/Fire™ 750 isotype control (right).
Compare all formats See APC/Fire™ 750 spectral data
Cat # Size Price Quantity Check Availability
152117 25 µg $159.00
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152118 100 µg $382.00
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Description

CD20 is a 33-37 kD protein, a member of the MS4A family, with four transmembrane spanning regions that present as a homo-oligomeric complexes in the cell surface when associating with MHC class I and II, CD53, CD81, and CD82. CD20 is expressed on B cells and a subset of T cells, but not on plasma cells. CD20 regulates B-cell activation and proliferation. Its ligation promotes transmembrane Ca2+ trafficking. CD20 is an important therapeutic target in the treatment of B cell lymphomas and leukemias.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD20 transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Bongard, A, et al. 2019. PLoS Pathog. 15(9):e1008043. (Depletion) Pubmed
RRID
AB_2910309 (BioLegend Cat. No. 152117)
AB_2910309 (BioLegend Cat. No. 152118)

Antigen Details

Structure
Member of the MS4A family, four transmembrane spanning regions, three isoforms of 37, 35, and 37 kD, depending on the phosphorylation degree.
Distribution

B cells and a subset of T cells

Function
Regulates B-cell activation and proliferation
Interaction
Associates with MHC class I and II, CD53, CD81, and CD82
Cell Type
B cells, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Morsy DE, et al. 2013. J Immunol. 191:3112-8.
  2. Lund FE and Randall TD. 2010. Nat Rev Immunol. 10:236-47.
  3. Beers SA, et al. 2010. Blood. 115:5191-201.
  4. Kuijpers TW, et al. 2010. J Clin Invest. 120:214-22.
Gene ID
12482 View all products for this Gene ID
UniProt
View information about CD20 on UniProt.org
Go To Top Version: 1    Revision Date: 03/01/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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