APC/Fire™ 810 anti-human CD64 Antibody

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Recombinant human CD64-Fc (1257-FC-050)

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 810 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 810 has a maximum excitation of 650 nm and a maximum emission of 810 nm.

Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect APC/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.

Excitation Laser

Red Laser (633 nm)

Application Notes

In-house testing suggests that S18012E partially blocks the binding of clone 10.1 but not clone S18012C. Clone 10.1 recognizes the EC3 epitope of CD64. While both CD64 subunits contain EC3 domains, clone 10.1 preferentially binds to CD64A (FcγRIA) but not CD64B (FcγRIB). 

RRID

AB_2904424 (BioLegend Cat. No. 399613)
AB_2904424 (BioLegend Cat. No. 399614)

Antigen Details

Structure

Ig superfamily, type I glycoprotein, 72 kD

Distribution

Monocytes, macrophages, dendritic cells, activated granulocytes

Function

Phagocytosis, ADCC

Ligand/Receptor

IgG receptor

Cell Type

Dendritic cells, Granulocytes, Macrophages, Monocytes

Biology Area

Immunology, Inhibitory Molecules

Molecular Family

CD Molecules, Fc Receptors

Antigen References

1. Hulett MD & Hogarth PM. 1994. Adv Immunol. 57:1-127. 
2. van de Winkel JG & Capel PJ. 1993. Immunol Today. 14:215-21. 

Gene ID

2209 View all products for this Gene ID

UniProt

View information about CD64 on UniProt.org

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Other Formats