- Regulatory Status
- RUO
- Other Names
- Streptavidin-Allophycocyanin, SAv-APC
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 405243 | 500 µg | $350.00 | |||
Streptavidin binds to biotin with high affinity. Streptavidin-APC is useful for detecting biotinylated antibodies. The excitation of APC by 600-635 nm laser light induces a fluorescence emission maximum of 660 nm.
Product Details
- Verified Reactivity
- Human, Mouse, Rat, All Species
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- Streptavidin is conjugated with APC under optimal conditions.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The Streptavidin-APC solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
ICFC - Verified
- Recommended Usage
-
Each lot of this Streptavidin-APC is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the APC conjugation. For immunofluorescent staining, the recommended use of this reagent is ≤ 0.06 µg per million cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Streptavidin-Allophycocyanin (APC) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-APC is 320 kD and Streptavidin alone is 52 kD.
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Application References
(PubMed link indicates BioLegend citation) -
- Kamimura D, et al. 2006. J. Immunol. 177:306.
- Andoniou CE, et al. 2005. Nature Immunology 6:1011.
- Chou WC,et al.2006.Blood108:3005. PubMed
- Malagolini N, et al. 2009. Glycobiology. 19:172. PubMed
- Kroeger KM, et al. 2009. J. Leukocyte Biol. PubMed
- Imada M, et al. 2009. Int. Immunol. 21:1151. PubMed
- Yi Y, et al. 2010 J. Biol Chem. 285:21233. PubMed
- King IL, et al. 2010. J. Immunol. 185:6138. PubMed
- Yokoyama T, et al. 2011. Int Immunol. PubMed
- Rusert P, et al. 2011. J. Exp Med. 208:1419. PubMed
- Bollig N, et al. 2012. PNAS. 109:8664. PubMed
- Nguyen V, et al. 2012. J. Immunol. 189:1081. PubMed
- Jager NA, et al. 2012. J Nucl Med. 53:1229. PubMed
- Liberman AC, et al. 2012. Mol Immunol. 50:220. PubMed
- Ogawa H, et al. 2012. Biochem Biophys Res Commun. 420:114. PubMed
- Ludin A, et al.2012. Nat Immunol. 13:1072. PubMed
- Sugiura D, et al. 2012. PLoS One. 7:e44770. PubMed
- Tani-Ichi S, et al. 2012. PNAS. 110:612. PubMed
- Jin L, et al. 2013. PNAS. 110:3907. PubMed
- Ota T, et al. 2013. J. Immunol. 191:3179. PubMed
- Fu X, et al. 2013. PNAS. 110:17982. PubMed
- Ryan SO, et al. 2014. Glycobiology. 24:362. PubMed
- Lyngaa R, et al. 2014. Clin Cancer Res. 20:1768. PubMed
- Pilling D, et al. 2014. PLoS One. 9:93730. PubMed
- Matthews JA, et al. 2014. PLoS One. 9:97707. PubMed
- Owen KA, et al. 2014. PLoS Pathog. 10:1004159. PubMed
- Duek A, et al. 2014. Blood. 123:3943. PubMed
- Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
- Product Citations
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Antigen Details
- Gene ID
- NA
- UniProt
- View information about Biotin on UniProt.org