- Clone
- CBR LFA-1/2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Integrin β2 subunit, LFA -1 β subunit, β2 integrin, ITGB2, b2 integrin, beta 2 integrin
- Isotype
- Mouse IgG1, κ
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Human peripheral blood lymphocytes were stained with CD18 (clone CBR LFA-1/2) APC/Fire™ 750 (filled histogram) or mouse IgG1, κ APC/Fire™ 750 isotype control (open histogram).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 366313 | 25 tests | $171.00 | |||
| 366314 | 100 tests | $375.00 | |||
CD18, also known as integrin β2 subunit, LFA-1 β subunit, and β2 integrin, is a 90 - 95 kD type I glycoprotein. CD18 non-covalently associates with CD11a, CD11b, or CD11c. CD18 is expressed on all leukocytes. CD18 and associated α chains function in the adhesion and signaling in hematopoietic cells.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Purified human LFA-1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.
- Excitation Laser
-
Red Laser (633 nm)
- Application Notes
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Additional reported applications (for the relevant formats of this clone) include: activation of LFA-1 and MAC-11,2. The LEAF™ or Ultra-LEAF™ purified antibody (Endotoxin < EU/µg, Azide-Free, 0.2 µm filtered) is recommended for function assays (contact our custom solutions team).
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Application References
(PubMed link indicates BioLegend citation) -
- Petruzzelli L, et al. 1995. J. Immunol. 155:854. (Activ)
- Lu C, et al. 2001. J. Immunol. 166:5629. (Activ)
- RRID
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AB_2721474 (BioLegend Cat. No. 366313)
AB_2721474 (BioLegend Cat. No. 366314)
Antigen Details
- Structure
- 90 - 95 kD type I transmembrane glycoprotein.
- Distribution
-
Leukocytes.
- Function
- Cell adhesion and signaling.
- Interaction
- Integrin, type I transmembrane glycoprotein, associates with CD11a, CD11b, or CD11c
- Ligand/Receptor
- ICAMs.
- Cell Type
- Leukocytes
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Anderson DC, et al. 1987. Annu. Rev. Med. 38:175.
2. Springer TA. 1994. Cell 76:301. - Gene ID
- 3689 View all products for this Gene ID
- UniProt
- View information about CD18 on UniProt.org
Other Formats
View All CD18 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD18 | CBR LFA-1/2 | FC |
| PE anti-human CD18 | CBR LFA-1/2 | FC |
| APC anti-human CD18 | CBR LFA-1/2 | FC |
| FITC anti-human CD18 | CBR LFA-1/2 | FC |
| PE/Cyanine7 anti-human CD18 | CBR LFA-1/2 | FC |
| Alexa Fluor® 647 anti-human CD18 | CBR LFA-1/2 | FC |
| APC/Fire™ 750 anti-human CD18 | CBR LFA-1/2 | FC |
| PerCP/Cyanine5.5 anti-human CD18 | CBR LFA-1/2 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD18
Human peripheral blood lymphocytes were stained with purifie... -
PE anti-human CD18
Human peripheral blood lymphocytes were stained with CD18 (c... -
APC anti-human CD18
Human peripheral blood lymphocytes were stained with CD18 (c... -
FITC anti-human CD18
Human peripheral blood lymphocytes were stained with CD18 (c... -
PE/Cyanine7 anti-human CD18
Human peripheral blood lymphocytes were stained with CD18 (c... -
Alexa Fluor® 647 anti-human CD18
Human peripheral blood lymphocytes were stained with CD18 (c... -
APC/Fire™ 750 anti-human CD18
Human peripheral blood lymphocytes were stained with CD18 (c... -
PerCP/Cyanine5.5 anti-human CD18
Human peripheral blood lymphocytes were stained with CD18 (c...