APC/Fire™ 750 anti-human IL-22 Antibody

Pricing & Availability
Clone
2G12A41 (See other available formats)
Regulatory Status
RUO
Other Names
IL22, IL-D110, IL-TIF, ILTIF, MGC79382, MGC79384, TIFIL-23, TIFa, zcyto18
Isotype
Mouse IgG2a, κ
2G12A41_APCFire750_IL-22_Antibody_051018
PMA and ionomycin (5 hours in the presence of Brefeldin A) stimulated human peripheral blood mononuclear cells were stained with CD4 FITC, fixed, permeabilized, and stained with APC/Fire™ 750 mouse IgG2a, κ isotype control (right), or APC/Fire™ 750 IL-22 (clone 2G12A41, left).
  • 2G12A41_APCFire750_IL-22_Antibody_051018
    PMA and ionomycin (5 hours in the presence of Brefeldin A) stimulated human peripheral blood mononuclear cells were stained with CD4 FITC, fixed, permeabilized, and stained with APC/Fire™ 750 mouse IgG2a, κ isotype control (right), or APC/Fire™ 750 IL-22 (clone 2G12A41, left).
Compare all formats See APC/Fire™ 750 spectral data
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366713 25 tests $159.00
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366714 100 tests $405.00
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Description

IL-22 is a cytokine that is structurally related to IL-10. Originally identified as a murine gene induced by IL-9 in T and mast cells, IL-22 was initially designated ILTIF, also known as the IL-10-related T cell-derived inducible factor. IL-22 belongs to a family of cytokines with limited homology specifically to IL-10, IL-19, IL-20, IL-24, IL-26, the IFN-λs, IL-28A, IL-28B, and IL-29. Human IL-22 shares 79% amino acid identity with murine IL-22 and 25% identity with human IL-10. IL-22 biological activity is initiated by binding to a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains. Its activity is further regulated through interactions with the soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1 (sIL-22R1). Both chains of the IL-22R complex belong to the class II cytokine receptor family. Two types of IL-22 binding receptors have been discovered: a membrane-bound receptor and a soluble receptor that are encoded by different genes. IL-22 is produced by Th17 cells and Th22 cells. The use of Iscove's Modified Dulbecco's Medium (IMDM) will result in better in vitro Th17 polarization. It plays a critical role in mucosal immunity in addition to the deregulated inflammation observed in autoimmune diseases.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant protein expressed in E. Coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.

Excitation Laser
Red Laser (633 nm)
RRID
AB_2734410 (BioLegend Cat. No. 366713)
AB_2734410 (BioLegend Cat. No. 366714)

Antigen Details

Structure
Cytokine.
Distribution
Thymic lymphomas, T cells, activated T cells, macrophages, mast cells, and Th17.
Function
Enhances the production of acute phase proteins in HepG2 cells and promotes Th17 cells to trasmigrate through the blood-brain barrier in multiple sclerosis.
Interaction
IL-22 receptor.
Ligand/Receptor
IL-22R1 and IL-10R2 receptor chains.
Cell Type
T cells, Macrophages, Th17, Mast cells, Embryonic Stem Cells
Biology Area
Immunology, Stem Cells
Molecular Family
Cytokines/Chemokines
Antigen References

1. Nagalakshmi ML, et al. 2004. Int. Immunopharmacol. 5:679.
2. Kebir H,, et al. 2007. Nat. Med. 13:1173.
3. Gu Y, et al. 2008. Eur. J. Immunol. 38:1807.
4. Pene Y, et al. 2008. J. Immunol. 180:7423.
5. Dumotier L, et al. 2000. J. Immune 164:1814.
6. Xie MH, et al. 2000. J. Biol. Chem. 40:31335.
7. Kotenko SV, et al. 2001. J. Immunol. 166:7096.
8. Chung Y, et al. 2006. Cell Res. 11:902.
9. Trifari S, et al. 2009. Nat. Immunol. 10:864.

Gene ID
50616 View all products for this Gene ID
UniProt
View information about IL-22 on UniProt.org
Go To Top Version: 1    Revision Date: 05/11/2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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