- Regulatory Status
- RUO
- Other Names
- 6CKine, Secondary lymphoid tissue chemokine (SLC), Exudos 2, Small inducible cytokine subfamily A, member 21 (SCYA21), SLC, TCA4, Small Inducible Cytokine Subfamily A (Cys-Cys) Member 21, Chemokine (C-C Motif) Ligand 21
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Human peripheral blood lymphocytes were surface stained with Alexa Fluor® 647 mouse IgG2a, κ isotype control (Cat. No. 400234) (left panel), Alexa Fluor® 647 anti-human CD197 (CCR7) (Cat. No. 353217) (middle panel), or with AZDye 647 Recombinant Human CCL21 (right panel). -
Biological activity was performed in HEK293T cells expressing human CCR7 by measuring its ability to recruit β-arrestin2. The ED50 for this effect was calculated at 0.5 to 3 µg/mL.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 598712 | 10 µg | $375.00 | |||
CCL21 is a CC chemokine highly expressed in lymph nodes. Although it shares only 32% identity with its paralog CCL19, these chemokines bind to the same receptor CCR7. CCL21 has a unique extension of about 30 amino acids with two extra cysteine residues in its carboxyl terminus. CCL21 has chemotactic effect for thymocytes and activated T cells, but not for B cells, macrophages and neutrophils. CCL21 is expressed in synovial tissue in rheumatoid arthritis (RA), and it has been suggested that CCL21, but not CCL19, induces human microvascular endothelial cell (HMVEC) migration and/or tube formation. In addition, it has been reported that sustained high concentrations of either soluble or immobilized CCL21 inhibit T cell proliferation and IL-2 secretion. CCL21 has been implicated with periapical periodontitis and scrapie
Product Details
- Source
- Human CCL21, amino acids Ser24-Pro134 (Accession # O00585) with a C-terminal AZDye647, was expressed in E. coli.
- Molecular Mass
- The 111 amino acid recombinant protein (Ser24-Pro134) has a predicted molecular weight of 14.2 kDa.
- Purity
- > 95%, as determined by mass spectrometry
- Formulation
- Lyophilized from 0.2 µm filtered solution of protein in DI-water.
- Concentration
- Lyophilized, 10 µg per vial
- Storage & Handling
-
Lyophilized at -20°C to -80°C until expiration
Lyophilized at 4°C for 6 months
Lyophilized at Room Temperature 1 month
Reconstituted at -20°C to -80°C for 12 months
Reconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted under aseptic conditions and stored at -20°C or colder. Stock solutions can be prepared at concentrations of 250 µg/mL up to 1 mg/mL in 0.22 µm filtered DI-water. Avoid repeated freeze/thaw cycles. - Activity
- Measured by its ability to recruit β-arrestin 2 in HEK293T cells transfected with human CCR7. The ED50 for this effect was measured at 0.5 to 3 µg/mL.
- Application
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FC, Live cell imaging
- Application Notes
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Protein has been verified for bioactivity prior to conjugation
Antigen Details
- Structure
- CCL21 is a 111 amino acids molecule with a predicted molecular weight of 14.2 kDa. PDB ID – 2L4N.
- Distribution
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CCL21 is highly expressed in HEV of lymph nodes and Peyer's patches. Also, it is expressed in the T cell zones of lymph nodes, in Peyer's patches by reticular cells, and in spleen by stromal cells. CCL21 is expressed by endothelial cells in lymphatic venules
- Function
- CCL21 chemoattracts naïve T cells and maturing dendritic cells (DCs) into T zones of secondary lymphoid organs. CCL21 and CCL19 chemoattract antigen-engaged B cells to the boundary of B and T zones to interact with T-helper cells. CCL19 and CCL21 inhibit apoptosis in DCs.
- Interaction
- Thymocytes, activated T cells, naïve CD4 and CD8, mature DCs, fibroblast-like synoviocytes of patients with rheumatoid arthritis (RA)
- Ligand/Receptor
- CCL21 binds to CCR7 receptor.
- Bioactivity
- Measured by its ability to recruit β-arrestin 2 in HEK293T cells transfected with human CCR7. The ED50 for this effect was measured at 0.5 to 3 µg/mL.
- Cell Type
- Dendritic cells, Endothelial cells, Leukocytes, Lymphocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells
- Biology Area
- Cell Biology, Immuno-Oncology, Immunology, Innate Immunity
- Molecular Family
- Adaptor Proteins, Cytokines/Chemokines, Innate Immune Signaling
- Antigen References
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- Hedrick JA & Zlotnik A. 1997. J Immunol. 159:1589-93.
- Reif K, et al. 2002. Nature. 416:94-9.
- Ziegler E, et al. 2007. J Immunol. 179:6485-93.
- Pickens SR, et al. 2012. Arthritis Rheum. 64:2471-81.
- Gene ID
- O00585 View all products for this Gene ID
- UniProt
- View information about CCL21 on UniProt.org