- Clone
- 7-14-4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
- Previously
-
Covance Catalog# MRB-5215
- Isotype
- Rabbit Ig
-
Direct ELISA showing specificity of Beta Amyloid 1-38 (7-14-4), Clone 7-14-4), on 1-38 (+ ctrl) and 1-40 (- ctrl) peptide coated plates.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 808603 | 50 µL | $475.00 | |||
Soluble Aβ (Amyloid-beta, beta-amyloid, or Abeta) peptides, 36 to 43 amino acids in length, are natural proteolytic cleavage products of amyloid precursor protein (APP). However, soluble abnormally aggregated Aβ species are associated with Alzheimer's disease (AD), can be toxic to neurons, and insoluble Aβ aggregates are the main component of cerebral amyloid plaques, which are a pathological hallmark of AD.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rabbit
- Formulation
- Phosphate-buffered solution.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Application
-
ELISA, WB, IHC
ICC - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by ELISA assay.
The optimal working dilution should be determined for each specific assay condition.
• WB: 1:1,000 - 1:2,000*
• IHC: 1:50 - 1:100
Tissue: Formalin-fixed Paraffin-embedded Tissues
Pre-treatment: 70% Formic Acid for 20 minutes at room temperature
Primary incubation: 24 hours at 2-4°C
• ELISA: Can be used as capture antibody in a sandwich ELISA with 4G8 detection (Ref Cat# 800707 Beta-Amyloid, 17-24 (4G8) Monoclonal Antibody, HRP Labeled, or Cat No. 800701 Beta-Amyloid, 17-24 (4G8) Monoclonal Antibody).
For capture antibody, recommended usage is between 1.0 and 10.0 µg/ml.
Recommended blocking buffer is 0.5-1.0% BSA in PBS.
If using Cat No. 800707 for detection; recommended usage is between 0.25-1.0 µg/ml. - Application Notes
-
This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC), and ELISA.
*Expected MW: 4 kD
WB Positive Control: Alzheimer's Brain Lysate
IHC Positive Control: Alzheimer's Brain Tissue
This antibody is reactive to the C-terminus of beta amyloid and is specific for the isoform that ends at the 38th amino acid. -
Application References
(PubMed link indicates BioLegend citation) -
- Watts JC, et al. 2014. Proc. Natl. Acad. Sci. USA 28:10323. ((F)
- Giaccone G, et al. 2012. Acta. Neuropathol. 124:809. (IHC, IF, WB)
- Product Citations
-
- RRID
-
AB_2564746 (BioLegend Cat. No. 808603)
- Disclaimer
-
Covered by US patents 5,675,063 and 7,429,487. Sold under license from Epitomics.
Antigen Details
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
- Molecular Family
- APP/β-Amyloid
- Gene ID
- 351 View all products for this Gene ID
- UniProt
- View information about beta-Amyloid 1-38 on UniProt.org
Other Formats
View All β-Amyloid, 1-38 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Biotin anti-β-Amyloid, 1-38 | 7-14-4 | ELISA,WB,IHC,ICC |
| Purified anti-β-Amyloid, 1-38 | 7-14-4 | ELISA,WB,IHC,ICC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Biotin anti-β-Amyloid, 1-38
Direct ELISA showing specificity of Beta Amyloid 1-38 (7-14-... -
Purified anti-β-Amyloid, 1-38